茶园芽孢杆菌qm7促生特性及耐酸铝机制初步研究分析.pdfVIP

茶叶科学 20 16，36 （6 ）：567~574 Journal of Tea Science 投稿平台： 茶园芽孢杆菌QM7 促生特性及耐酸铝机制初步研究 罗毅，苏有健，张永利，夏先江，宋莉，王烨军，廖万有* 安徽省农业科学院茶叶研究所，安徽 黄山 245000 摘要：目前含芽孢杆菌等微生物制剂的有机肥料在酸性茶园土壤中的生物活性不高，难以起到有效的促生作 用，达到部分替代和减少化肥使用量的目的。本实验分离得到 1 株耐酸铝芽孢杆菌，命名为 QM7 。试验发现 菌株促生能力受到铝离子浓度的影响，在无铝条件下菌株生长素（IAA ）的分泌量为 85.6 mg·L- 1 ，当铝离子 浓度为 1 mmol ·L- 1 时IAA 分泌量为36.8 mg·L- 1 ；盆栽结果表明，在6 周内菌株可以增加茶苗根系表面积56.8% ， 根尖数 27.3% ；蛋白电泳分析发现高浓度的铝胁迫会导致菌株胞内 64 种与转录、翻译和代谢相关蛋白的表达 发生差异，使得菌株生长受到抑制。 关键词：茶园土壤；耐酸铝芽孢杆菌；生长素（IAA ）；蛋白电泳 中图分类号：S154.3 ；Q939.1 文献标识码：A 文章编号：1000-369X （2016 ）06-567-08 Research on The Promoting Effects on Tea Growth and Aluminum Tolerant Mechanism of Bacillus subtilis Strain QM7 * LUO Yi, SU Youj ian, ZHANG Yongli, XIA Xianjiang, SONG Li, WANG Yejun, LIAO Wangyou Tea Research Institute, Anhui Academy of Agricultural Sciences, Huangshan 245 000, China Abstract: At present, the biological activities of Bacillus .spp and other microorganisms in the acid tea garden soil are not high, thereby difficult to effectively promote plant growth and replace or reduce the usage of chemical fertilizer. In this study, one of Aluminum (Al) tolerance strains, Bacillus subtilis QM7 was isolated from tea garden soil. The auxin (IAA) production of QM7 was influenced by Al concentration (from 85.6 mg·L- 1 to 36.8 mg·L- 1 under 0 to 1 mmol ·L- 1 external Al respectively). Results of pot experiment showed that strain QM7 could promote plant growth by increasing root surface area by 56.8% and numbers of root tips by 27.3% in 6 weeks. Two -dimen