山苦瓜萃取物活化转录因子PPARγ及抑制巨噬细胞发炎-亚洲大学.PDF

山苦瓜萃取物活化轉錄因子PPARγ及抑制巨噬細胞發炎介質之分泌 1 1* 周友民 、趙哲毅 Wild Bitter Gourd Extracts Activate the Transcription Factor PPARγ and Inhibit LPS-Induced Inflammatory Responses in RAW264.7 5 Cells 1 1* Yeou-Min Chou , Che-Yi Chao 1 亞洲大學保健營養生技學系 1Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan, ROC 10 Running Title : 山苦瓜萃取物對發炎反應的影響 通訊作者： 趙哲毅 博士，亞洲大學保健營養生技學系，41354台中縣霧峰鄉柳豐路 500號 15 Corresponding author: Che-Yi Chao, Ph.D., Department of Health and Nutrition Biotechnology, Asia University, No. 500, Lioufeng Rd., Wufeng, Taichung County 41354, Taiwan (R.O.C.) Tel: 886 + (0)4 + 2332-3456 ext. 1859 E-mail: cychao@asia.edu.tw 1 Wild Bitter Gourd Extracts Activate the Transcription Factor PPARγ and Inhibit LPS-Induced Inflammatory Responses in RAW264.7 Cells ABSTRACT 5 Recently, the investigation showed that bitter gourd extracts could activate the transcription factor peroxisome proliferator-activated receptors (PPARs) and regulate the target genes expression. The ethyl acetate extracts (EAE) of wild bitter gourd (Momordica charantia L. var. abbreviata Seringe) including stem, leaf, flower and 10 fruit residue and the water soluble extracts were used to study the inhibition of the LPS-induced inflammatory responses in RAW264.7 cells. RAW264.7 macrophage cells were treated with different parts of wild bitter gourd extracts (10, 50,100 μg/