原创力文档a method for the generation of ectromelia virus (ectv) recombinants in vivo analysis of ectv vcd30 deletion mutants方法一代的畸形病毒(ectv)重组的体内分析ectv vcd30缺失突变体.pdfVIP
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a method for the generation of ectromelia virus (ectv) recombinants in vivo analysis of ectv vcd30 deletion mutants方法一代的畸形病毒(ectv)重组的体内分析ectv vcd30缺失突变体
A Method for the Generation of Ectromelia Virus (ECTV)
Recombinants: In Vivo Analysis of ECTV vCD30 Deletion
Mutants
1 2¤a ˜ ¨ 1 3 ´
Ali Alejo , Margarida Saraiva , Maria Begona Ruiz-Arguello , Abel Viejo-Borbolla , Mar Fernandez de
Marco3¤b, Francisco Javier Salguero1¤c, Antonio Alcami2,3*
´ ´ ´
1 Centro de Investigacion en Sanidad Animal, Instituto Nacional de Investigacion y Tecnologıa Agraria y Alimentaria, Valdeolmos, Madrid, Spain, 2 Department of
´
Medicine, University of Cambridge, Addenbrooke’s Hospital, Cambridge, United Kingdom, 3 Centro de Biologıa Molecular Severo Ochoa (Consejo Superior de
´ ´
Investigaciones Cientıficas and Universidad Autonoma de Madrid), Cantoblanco, Madrid, Spain
Abstract
Background: Ectromelia virus (ECTV) is the causative agent of mousepox, a lethal disease of mice with similarities to human
smallpox. Mousepox progression involves replication at the initial site of infection, usually the skin, followed by a rapid
spread to the secondary replicative organs, spleen and liver, and finally a dissemination to the skin, where the typical rash
associated with this and other orthopoxviral induced diseases appears. Case fatality rate is genetically determined and
reaches up to 100% in susceptible mice strains. Like other poxviruses, ECTV encodes a number of proteins with
immunomodulatory potential, w
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