三种采血模式血液分析仪测定结果分析.docVIP

三种采血模式血液分析仪测定结果分析[摘要] 目的:探讨静脉防凝全血、手指末梢防凝全血和手指末梢稀释血三种采血模式在全自动血液分析仪上血细胞测定的结果差别。方法:肘静脉血、手指末梢血用EDTA-K2防凝,手指末梢血用稀释液1∶26稀释,分别用全血模式和稀释模式测定结果。结果:肘静脉防凝全血、手指末梢防凝全血、手指末梢稀释血三者的白细胞(WBC)、红细胞(RBC)、血红蛋白(HCG)、红细胞比容(HCT)的结果均无显著性差异,但在血小板(PLT)结果中,前两种防凝血结果差异无统计学意义,而均与手指末梢稀释血结果有显著性差异。结论:在一定条件下,可用手指末梢防凝全血替代肘静脉防凝全血进行血液测定;手指末梢稀释血结果不稳定,不建议使用。 [关键词] 静脉血;末梢血;血液分析仪;结果分析 [中图分类号]R446.11+3 [文献标识码] B[文章编号] 1673-7210(2009)07(a)-116-02 Analysis of three blood collection models of hematology analyzer HE Lei (Department of Laboratory, Laigang Group Hospital, Shandong Province, Laiwu 271126, China) [Abstract] Objective: To investigate the difference of three blood collection models that are determined by automatic hematology analyzer. Methods: Venous blood, peripheral blood from finger was treated with anticoagulant(EDTA-K2) to prevent clotting of blood; meanwhile, peripheral blood from finger was diluted by dilute liquid(1∶26). The blood samples were analyzed with whole blood model and dilute model respectively. Results: White blood cell(WBC), red blood cell (RBC), hemoglobin(HCG) and hematocrit (HCT) in three models showed no significant difference, but platelet count (PLT) showed significant difference between the peripheral blood of diluted and anticoagulant venous blood or anticoagulant peripheral blood. Conclusion: Under a certain condition, it is feasible that anticoagulant peripheral blood replaces anticoagulant venous blood, the results of peripheral blood been diluted are unsteady, and it is not suggested to put into use. [Key words] Venous blood; Peripheral blood; Hematology analyzer; Result analysis 在全自动血液分析仪上进行血细胞测定时,一般有两种模式:全血模式和稀释模式。由于稀释模式采的是手指末梢血液,在采血过程中产生的误差因素很多,结果不稳定,因而现在多采用静脉全血,但用于某些特殊患者时,如新生儿、婴幼儿、大面积烧伤、多次放疗、化疗的恶性肿瘤患者,往往采集静脉血有困难,患者更易接受采末梢血。对于静脉全血与末梢稀释血的比较多有报道,而对静脉全血与末梢防凝全血的结果比较鲜有研究。本文探讨在上述情况下用手指末梢防凝全血替代肘静脉防凝全血进行血液测定,同时进行了三种采血模式的结果比较。现报道如下: 1 材料与方法 1.1 仪器 日本Sysmex KX-21血液分析仪。 1.2 试剂 KX-21配套溶血剂、稀释液(日本Sysmex公司);血球质控液:南京普朗公司PHC-3D血细胞质控物(批号411081);防凝管