细粒棘球绦虫棘球蚴病动物模型制备的探索-第三军医大学学报.DOC

异源接种建立小鼠和兔包虫病动物模型(张静1,2，叶彬1,2，邹晓毅1,2，武卫华1,2，韩秀敏3 摘要：目的 人工接种羊源细粒棘球绦虫原头蚴感染法建立小鼠与兔包虫病动物模型。 方法 6周龄昆明小鼠经皮穿刺腹腔内接种羊源细粒棘球绦虫原头蚴悬液，新西兰大白兔于腹部手术后肝脏接种羊源细粒棘球绦虫原头蚴悬液。接种原头蚴6个月后剖检动物，观察小鼠腹腔和新西兰大白兔肝脏内包虫囊生长情况。结果 接种羊源细粒棘球绦虫原头蚴6个月后，小鼠腹腔内包囊生成率为95%，新西兰大白兔肝内包囊生成率为5%。光镜观察见在小鼠腹腔和兔肝脏形成的囊泡具有与羊肝脏棘球蚴囊壁类似的类上皮细胞层和板层状，而囊壁厚度较羊肝脏棘球蚴囊壁薄。结论 以羊源细粒棘球绦虫原头蚴悬液接种昆明小鼠腹腔和新西兰大白兔肝脏，可以建立小鼠和兔包虫病动物模型 关键词：异源接种；包虫病；动物模型 R383.33 Heterogenetic Inoculative Method For Establishing Laboratory Animal Models of Hydatid Disease in Mice and Rabbits Zhang Jing1,2，Ye Bin1,2， Zou Xiaoyi1,2，Wu Weihua1,2，Han Xiuming3 Department of Chongqing Medical University, Chongqing 400016, China) Research Center for Molecular Medicine and Tumor, Chongqing Medical University, Chongqing 400016, China) （3. Qinghai Institute for Endemic Diseases Prevent and Control, Xining 811602,China） Abstract: [Objective] To establish laboratory animal models for hydatid disease of mice and rabbits. [Methods] The 6-weeks-old Kunming mice were inoculated in abdomen with the suspensions (0.5ml saline containing 2000 protoscolice isolated from the hyditads in sheep livers, 1000U Penicillin and 1000U Streptomycin). The New Zealand rabbits, whose livers were exposed after a surgical operation of abdominal region, were inoculated in live with 0.5ml suspensions of 4000 above mentioned protoscolice. The hydatid cysts in the animals were observed by naked eye and light microscope 6 months later after the inoculation experiments. [Results] On the observation time point, the hydatid cysts were observed in the abdominal cavity of 95% experimental mice (19/20), but in the liver of 50% experimental rabbits (1/2) respectively. The cyst wall structures of hydatid derived from the experimental mice and rabbit were observed the characteristic structures with a single layer germinal cell and lamellar cell free zone, which were similar to hydatids derived from the sheep liver but with a less thick cyst wall. The protoscolex were found in the hydatid cysts derived from the thir