不同方法提取鲢鱼皮胶原蛋白的理化特性比较.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.5 不同方法提取鲢鱼皮胶原蛋白的理化特性比较 张强，王倩倩，陆剑锋，吕顺，叶应旺，姜绍通，林琳 （合肥工业大学生物与食品工程学院，安徽合肥 230009） 摘要：本文分别采用酸法（ASC ）、酶法（PSC ）和热水浸提法（HWSC ）提取鲢鱼皮胶原蛋白，考察不同提取方法对鱼皮胶原 蛋白理化性质的影响。研究表明，3 种方法所提取的鱼皮胶原蛋白的紫外吸收光谱、红外吸收光谱都较为相似，在 232 nm 处均出现 胶原蛋白的最大吸收峰，在 280 nm 处几乎无吸收；SDS电泳图谱显示3 种方法所提取的胶原蛋白，其亚基组成形式均为(α) α ， 1 2 2 同时，3 种方法所提取的胶原蛋白其氨基酸组成和比例类似，均符合 Ⅰ型胶原蛋白特征。热水浸提法所提取的鱼皮胶原蛋白（HWSC ） 羟脯氨酸含量较低，凝胶特性较好，酸法提取对胶原蛋白空间网络结构影响最小。ASC 、PSC 和 HWSC 的热变性温度分别为 31.05±0.14 ℃、31.45±0.01 ℃、43.75±1.20 ℃。不同提取方法对鱼皮胶原蛋白的羟脯氨酸含量、流变学特性、热变性温度及微观结构 有一定的影响。 关键词：白鲢鱼皮；胶原蛋白；提取；理化性质；比较 文章篇号：1673-9078(2014)5-104-110 Comparison of Physical and Chemical Characteristics of Collagen from Silver Carp Skin using Different Extraction Methods ZHANG Qiang, WANG Qian-qian, LU Jian-feng, LV Shun, YE Ying-wang, JIANG Shao-tong, LIN Lin (School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China) Abstract: Acid soluble collagen (ASC), pepsin soluble collagen (PSC) and hot water-soluble collagen (HWSC) were successfully extracted from silver carp skin, and the physical and chemical characteristics of them were investigated and compared. The results showed that UV and FT-IR measurements of ASC, PSC and HWSC were quite similar, and all of them showed maximum absorption peak at 232 nm and almost no absorption peak at 280 nm. SDSpattern indicated that all the collagen extracted by three methods contained two different α chains -(α) α, and their amino acid composition and proportion were similar. It was conformed to the characteristics of type I collagen.