a method for serial tissue processing and parallel analysis of aberrant crypt morphology, mucin depletion, and beta-catenin staining in an experimental model of colon carcinogenesis串行组织处理和并行的方法分析异常的隐窝形态、粘蛋白耗竭,β-连环蛋白染色的实验模型结肠致癌作用.pdfVIP

Biol Proced Online (2010) 12:118– 130 DOI 10.1007/s12575-010-9032-x A Method for Serial Tissue Processing and Parallel Analysis of Aberrant Crypt Morphology, Mucin Depletion, and Beta-Catenin Staining in an Experimental Model of Colon Carcinogenesis John N. McGinley Matthew D. Thompson Henry J. Thompson Received: 11 March 2010 /Accepted: 23 April 2010 /Published online: 18 May 2010 # The Author(s) 2010. This article is published with open access at S Abstract The use of architectural and morphological Keywords colon carcinogenesis . aberrant crypt foci . characteristics of cells for establishing prognostic indicators mucin depletion . beta-catenin . image analysis . by which individual pathologies are assigned grade and morphometry stage is a well-accepted practice. Advances in automated micro- and macroscopic image acquisition and digital Abbreviations image analysis have created new opportunities in the field ACF aberrant crypt foci of prognostic assessment; but, one area in experimental GALT gut-associated lymphoid tissue pathology, animal models for colon cancer, has not taken HE hematoxylin and eosin advantage of these opportunities. This situation is primarily HID-AB high iron diamine–alcian blue due to the methods available to evaluate the colon of the MDF mucin-depleted foci rodent for the presence of premalignant and malignant PBS phosphate-buffered saline pathologies. We report a new method for the excision and TBS Tris-buffered saline processing of the entire colon of the rat and illustrate how this procedure permitted the quantitative assessment of aberrant crypt foci (ACF), a premalignant colon pathology, for characteristics consistent with progression to malignancy. 1 Introdu