a method to study the epigenetic chromatin states of rare hematopoietic stem and progenitor cells; minichip–chip一个方法来研究表观遗传罕见的造血干细胞和祖细胞的染色质状态;.pdfVIP

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a method to study the epigenetic chromatin states of rare hematopoietic stem and progenitor cells; minichip–chip一个方法来研究表观遗传罕见的造血干细胞和祖细胞的染色质状态;.pdf

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a method to study the epigenetic chromatin states of rare hematopoietic stem and progenitor cells; minichip–chip一个方法来研究表观遗传罕见的造血干细胞和祖细胞的染色质状态;

Biol Proced Online (2010) 12:1– 17 DOI 10.1007/s12575-010-9031-y A Method to Study the Epigenetic Chromatin States of Rare Hematopoietic Stem and Progenitor Cells; MiniChIP–Chip Holger Weishaupt Joanne L. Attema Received: 25 March 2010 /Accepted: 21 April 2010 /Published online: 15 May 2010 # The Author(s) 2010. This article is published with open access at S Abstract Dynamic chromatin structure is a fundamental 1 Introduction property of gene transcriptional regulation, and has emerged as a critical modulator of physiological processes during A unique and defining property for any stem cell cellular differentiation and development. Analysis of chro- population is intrinsic self-renewal throughout the process matin structure using molecular biology and biochemical of cell division, whilst maintaining the capacity to form assays in rare somatic stem and progenitor cells is key for multiple differentiated and mature cell types over the lifetime understanding these processes but poses a great challenge of an organism. Stem cells undergo dramatic changes in because of their reliance on millions of cells. Through the morphology, cell cycle status and gene expression during development of a miniaturized genome-scale chromatin differentiation into specialized progenitor subsets. Such immunoprecipitation method (miniChIP–chip), we have alterations are proposed to result from chromatin reorganiza- documented the genome-wide chromatin states of low tion of the genome, allowing for the establishment and abundant populations that comprise hematopoietic stem cells maintenance of lineage-specific transcriptional networks [ 1, and immediate progeny residing in murine bone marrow. In 2]