an irgd based strategy to study electrochemically the species inside a cell基于irgd策略研究电化学物种在一个细胞.pdfVIP

Int. J. Mol. Sci. 2012, 13, 10424-10431; doi:10.3390/ijms130810424 OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article An iRGD Based Strategy to Study Electrochemically the Species Inside a Cell Limin Ning 1, Xiaoxi Li 1, Xiaorong Ding 2,3, Yongmei Yin 3 and Genxi Li 1,2,* 1 Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China; E-Mails: ninglimin2006@126.com (L.N.); lxx.zlxhaoyou@ (X.L.) 2 Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China; E-Mail: dingxiaoronglaofuzi@126.com 3 Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; E-Mail:ym.yin@ * Author to whom correspondence should be addressed; E-Mail: genxili@; Tel.: +86-25-8359-3596; Fax: +86-25-8359-2510. Received: 15 May 2012; in revised form: 27 June 2012 / Accepted: 9 August 2012 / Published: 21 August 2012 Abstract: This paper reports a method for electrical communication between the inner part of cells and an electrode with the help of iRGD peptide. Due to the enhancement of the cell penetration caused by iRGD peptide, DNA molecules, previously modified on a gold electrode surface, can be easily transfected into the cells. At the same time, doxorubicin, an anticancer drug, can also be transfected into cells with high penetra