apoptosis inducing effect of plumbagin on colonic cancer cells depends on expression of cox-2细胞凋亡诱导plumbagin对结肠癌细胞的影响取决于cox - 2的表达.pdfVIP

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apoptosis inducing effect of plumbagin on colonic cancer cells depends on expression of cox-2细胞凋亡诱导plumbagin对结肠癌细胞的影响取决于cox - 2的表达.pdf

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apoptosis inducing effect of plumbagin on colonic cancer cells depends on expression of cox-2细胞凋亡诱导plumbagin对结肠癌细胞的影响取决于cox - 2的表达

Apoptosis Inducing Effect of Plumbagin on Colonic Cancer Cells Depends on Expression of COX-2 1 1 1 Bharathi Raja Subramaniya , Gayathri Srinivasan , Sakeena Sadullah Mohammed Sadullah , Nimitha 1 3 2 1 Davis , Lakshmi Baddi Reddi Subhadara , Devaraj Halagowder , Niranjali Devaraj Sivasitambaram * 1 Department of Biochemistry, School of Life Sciences, University of Madras, Guindy Campus, Chennai, Tamilnadu, India, 2 Department of Zoology, School of Life Sciences, University of Madras, Guindy Campus, Chennai, Tamilnadu, India, 3 Centre for Biotechnology, Anna University, Chennai, Tamilnadu, India Abstract Plumbagin, a quinonoid found in the plants of the Plumbaginaceae, possesses medicinal properties. In this study we investigated the anti-proliferative and apoptotic activity of plumbagin by using two human colonic cancer cell lines, HT29 and HCT15. IC50 of Plumbagin for HCT15 and HT29 cells (22.5 mM and 62.5 mM, respectively) were significantly different. To study the response of cancer cells during treatment strategies, cells were treated with two different concentrations, 15 mM, 30 mM for HCT15 and 50 mM, 75 mM for HT29 cells. Though activation of NFkB, Caspases-3, elevated levels of TNF-a, cytosolic Cytochrome C were seen in both HCT15 cells HT29 treated with plumbagin, aberrant apoptosis with decreased level of pEGFR, pAkt, pGsk-3b, PCNA and Cyclin D1was observed only in 15 mM and 30 mM plumbagin treated HCT15 and 75 mM plumbagin treated HT29 cells. This suggests that plumbagin induces apoptosis in both HCT15 cells and HT29 treated, whereas, proliferation was inhibited on