口腔癌患者外周血树突状细胞生物学特性.docVIP

口腔癌患者外周血树突状细胞生物学特性 　 作者：张素欣，王士杰，单保恩，段玉芹，许艳枝 【关键词】 口腔肿瘤;树突状细胞;表型；增殖 　　Biological characteristics of monocytederived dendritic cells from peripheral blood of patients with oral carcinoma 　　【Abstract】 AIM: To investigate the morphology, phenotype and functions of dendritic cell (DC) from peripheral blood of the healthy individuals and the patients with oral carcinoma. METHODS: Fifteen patients with oral carcinoma at stages of II or above were assigned to study group and 15 healthy volunteers to control group. Monocytes were isolated by density centrifugation, and cultured in the presence of GMCSF, IL4 and TNFα for 7 d in vitro. The morphological change of the cultured cells was observed by light microscopy and electron microscopy. The expressions of CD83, CD1a, CD86, CD80, HLADR were analyzed by flow cytometry. The ability of DC to stimulate the proliferation of lymphocyte in mixed lymphocyte reactions (MLR) was determined with the method of MTT. RESULTS: Morphologically and phenotypically typical DC was obtained in the 2 groups after culture for 7 d. The expression rates of CD83, CD1a on DC from the patients were 49.3±9.5 and 48.1±7.3 respectively，significantly lower than those from healthy volunteers (Plt;0.05). The DC from the patients had the lower expression levels of CD86, CD80 and HLADR (85.5±8.7, 83.2±8.1 and 78.3±5.4) than healthy volunteers did (89.2±6.7, 87.7±7.2 and 82.3±4.1), but not significantly (Pgt;0.05). Moreover, the mean fluorescence intensity of CD83, CD1a, CD80, CD86 and HLADR expressed in study group were 4.80±2.13, 3.96±1.15, 19.63±8.12, 12.33±6.75 and 39.44±7.9, respectively, which were lower but not statistically different from the control group (Pgt;0.05). In addition, DC from the study group had the ability to stimulate the proliferation of allogeneic and autogenous lymphocytes in vitro. CONCLUSION: Monocytes in peripheral blood of the patients with oral carcinoma can be induced to differentiate into mature DC with the ability to stimulate