抑肽酶对犬肺移植中缺血-再灌注损伤作用.doc

抑肽酶对犬肺移植中缺血/再灌注损伤作用 　 作者：田丰，程庆书，李小飞，王小平，姜涛，周勇安，杨晔，陈连宏 【关键词】 肺移植 　　Effect of aprotinin on lung ischemia/reperfusion injury in lung transplantation in canines 　　【Abstract】 AIM： To evaluate the effect of aprotinin on the ischemia/reperfusion injury of the lung in lung transplantation in canines. METHODS: Twenty male canines were randomly divided into two groups. In each group the canines were randomly assigned to donors (n=5) and recipients (n=5). The recipients received either aprotinin 25 U/kg iv. infusion (Group I) or equivalent volumes of 50 g/L glucose i.v.(Group II) before reperfusion. The lung tissue samples were obtained one hour after reperfusion. The pulmonary water content, tissue myeloperoxidase (MPO) activity, content of MDA and nitric oxide (NO) were measured. Microscopic examination of lungs was also conducted. RESULTS: The pulmonary water content (7365±431)%, the tissue MPO activity (021±005 ΔOD/min#12539;g), the content of MDA (017±0004 nmol/mg#12539;prot) and the content of NO (1260±873 nmol/mg#12539;prot) in group I were significantly lower respectively than those in control (8133±367)%, (037±010 ΔOD/min#12539;g), (029±0010 nmol/mg#12539;prot) and (3509±1373 nmol/g#12539;prot) (Plt;005). The microscopic examination showed that there were severe leukocyte infiltration and edema formation in the alveolar space in group II, but the changes were less severe in group I. CONCLUSION: The application of aprotinin before reperfusion can protect the lung against ischemia/reperfusion injury in lung transplantation in canine. 　　【Keywords】 lung transplantation; aprotinin;reperfusion injury 　　【摘要】 目的： 研究抑肽酶(aprotinin)在犬肺移植手术中对肺组织缺血/再灌注损伤的作用.方法： 20只成年雄性犬随机分为两组，每组供体和受体各5只.建立肺移植动物模型.I组（抑肽酶组）： 术中受体给予aprotinin静滴（25 u/kg），II组（对照组）： 给予等容积50 g/L葡萄糖溶液.恢复血流灌注1 h后，取移植肺组织为标本，检测肺组织中丙二醛（MDA）含量、髓过氧化物酶（MPO）活力、一氧化氮（NO）含量、肺组织含水量并做HE染色光镜下观察病理变化.结果： aprotinin组的肺组织含水量(7365±431)%较对照组(8133±367)%显著降低（P＜005）.aprotinin组肺组织中髓过氧化物酶活性(021±005 ΔOD/ min#12539;g)显著(P＜005)低于对照组(037±010 ΔOD/min#1