正交设计法优选戒毒胶囊提取工艺.docVIP

正交设计法优选戒毒胶囊提取工艺 　 作者：靳怡然，杜英峰，袁志芳，张兰桐 【摘要】 　　目的优选戒毒胶囊中挥发油、醇及水提取工艺。方法采用正交设计法，以挥发油得率为指标，筛选制剂中挥发油的提取工艺；以黄芩苷的含量为考察指标筛选醇提取工艺；以芍药苷的含量为考察指标优选金银花、白芍、黄芪等药味的水提工艺。结果挥发油的最佳提取工艺为当归、川芎加水10倍量，浸泡1 h，提取8 h；醇提最佳工艺为丹参、黄芩、栀子采用70%乙醇，溶媒6倍量，回流时间为1.5h，提取次数为2次；水提取的最佳工艺为将白芍、黄芪、龟甲和熟地、黄连同上述药渣，加10倍量水，煎煮2次，1.5 h/次，金银花于第2次煎煮时加入。结论通过正交实验优选出戒毒胶囊最佳制备工艺稳定可行。 【关键词】 正交设计； 戒毒胶囊； 提取工艺 　　Abstract：ObjectiveTo optimize the extraction process of Jiedu Capsules.MethodsThe series of tests were carried out with orthogonal design. The conditions were evaluated with the yield of volatile oil as the index. With the yield rate of baicalin as the index, and the concentration of alcohol, solvent volume, extracting time and times as factors, the optimal extracting process for the extracting with alcohol was studied. With the yield rate of paeoniflorin as the index, and the volume of water, extracting time and times were optimized for the water extraction. ResultsThe optimal extracting process of volatile oil was as follows: 10 volumes of water were added and immersed for 1h, and then extracted for 8h. The results of alcohol extraction was defined as 85% of alcohol, 1.5 hours of refluxing time with 10 times volume of solvent and twice extraction. The best extracting process by water was defined as 1.5 hours of each extraction with 10 times volume of water and twice extraction. Flos Lonicerae was extracted at the second extraction.ConclusionThe optimum preparation technology of Jiedu Capsule is suitable and reproducible. 　　Key words：Chinese traditional medicine; Orthogonal design; Extracting process 　　戒毒胶囊的处方为治疗吸食阿片类成瘾者的临床经验方，由金银花、黄芩、栀子、丹参、黄芪等13味药制成。本实验根据戒毒胶囊处方组成和各药味之间的关系，在参考各药物所含成分的理化性质和药理作用的研究基础上［1，2］，根据与治疗作用相关的有效成分或有效部位的理化性质，结合制剂制备上的要求等实际情况，采用正交设计法对工艺路线的设计，筛选了工艺方法和条件，制定出方法简便、条件确定的稳定工艺。 　　1 仪器与试药 　　1.1 仪器挥发油提取器（上海求精玻璃仪器厂）；美国Waters高效液相色谱仪，510泵，2487紫外检测器；色谱工作站（Empower色谱工作站）；TCQ-250超声波清洗器（北京医疗设备二厂）。 　　1.2 试药黄芩苷、绿原酸、芍药苷对照品均购自中国药品生物制品检定所；药材均购自石家庄市药材站，经河北医科大学生药学教研室鉴定。乙腈为色谱纯，水为重蒸水，其余试剂均为分析纯。 　　2 方法与结果 　　2.1 挥发油的提取工艺［3］ 　　2