正常及6羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激反应.docVIP

正常及6羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激反应 　【摘要】 目的 在体水平上观察选择性胆碱能M受体激动剂氧化震颤素(OxoM)对正常和6羟基多巴胺(6OHDA)毁损大鼠脚桥核(PPN)神经元自发电活动的影响。方法 采用玻璃微电极细胞外记录和PPN内注射OxoM，观察PPN神经元电活动的变化。结果 与正常组大鼠相比，6OHDA毁损组大鼠PPN神经元的平均放电频率增高(Plt;0.01)，神经元活动趋于不规则(Plt;0.001);PPN内注射OxoM对正常组和6OHDA毁损组大鼠的PPN神经元放电频率均可产生升高、降低和无变化三种改变。然而，PPN内注射OxoM对正常组大鼠PPN神经元的平均放电频率无明显影响，但OxoM使6OHDA毁损组大鼠PPN神经元的平均放电频率显著降低(Plt;0.001)。结论 胆碱能M受体激动剂抑制损毁组大鼠PPN神经元的活动，其作用机制是复杂的，涉及到PPN内突触前和突触后胆碱能受体。 【关键词】 脚桥核;M型胆碱能受体;氧化震颤素;帕金森病;电生理学 　　 ABSTRACT: Objective To explore the effects of oxotremorine M (OxoM), muscarinic acetylcholine receptor agonist, on spontaneous firing rate of neurons of pedunculopontine nucleus (PPN) in normal and 6hydroxydopamine (6OHDA)lesioned rats.Methods Change in the firing activity of PPN neurons was observed by extracellular recording in vivo and local injection of OxoM in the PPN.Results The results showed that the mean firing rate of PPN neurons in the lesioned rats was significantly increased compared to that in normal rats (Plt;0.01), and that the firing pattern of the neurons changed toward a more irregular firing after the lesion (Plt;0.001). IntraPPN injection of OxoM induced increase, decrease or no change in the firing rate of PPN neurons in both the normal and 6OHDAlesioned rats. However, the mean firing rate of PPN neurons in normal rats remained unaltered by the stimulation of M receptors, but the mean firing rate of PPN neurons in the lesioned rats decreased significantly by intraPPN injection of OxoM (Plt;0.001).　Conclusion M receptor agonist inhibits the activity of the PPN neurons in lesioned rats; the action mechanism of the agonist is complicated, involving not only postsynaptic but also presynaptic M receptors located in different sites within the PPN. 　　KEY WORDS: pedunculopontine nucleus; M acetylcholine receptor; oxotremorine; Parkinsons disease; electrophysiology 　　脚桥核(pedunculopontine nucleus, PPN)是一中脑的神经结构，由胆碱能和非胆碱能神经元组成[1]。解剖学和电生理学研究表明PPN与基底神经节有丰富的交互纤维联系，在运动调控中起重要作