生姜油预防日本血吸虫尾蚴感染实验探究.docVIP

生姜油预防日本血吸虫尾蚴感染实验探究 　 作者：边藏丽 夏鹏 黄柏青 金露萍 李巧 【摘要】 　　目的探讨生姜油预防日本血吸虫尾蚴感染的效果以及对皮肤的毒副作用。 方法将实验小鼠随机分为A、B、C 3组，先将生姜油涂抹于A、B组小鼠皮肤， A组小鼠直接用血吸虫尾蚴感染，B组小鼠用泥浆水洗擦后感染血吸虫尾蚴，C组为对照组，A、B两组结果与对照组比较，观察生姜油预防血吸虫尾蚴感染的效果。另以生姜油涂于小鼠腹部, 观察对皮肤的毒副作用。结果A组小鼠血吸虫尾蚴的感染率为0，减虫率达100%； B组小鼠感染率为10%，减虫率达88.5%；两组结果与对照组比较血吸虫尾蚴感染率差异均有显著统计学意义（P＜0.001）。小鼠皮肤涂生姜油后未见异常反应。结论生姜油涂肤有防御日本血吸虫感染的作用；对皮肤无明显毒副作用。 【关键词】 生姜油； 日本血吸虫； 尾蚴； 小鼠 　　Abstract：ObjectiveTo investigate the preventive effects of Ginger oil against the infection caused by Schistosoma japonicum cercariae and its toxic and side effects to skin. MethodsThe mice were divided into Ginger oil group (group A), muddy water group (group B) and control group (group C) at random. Ginger oil was besmeared on the skin of mice and then the mice were either directly infected with Schistosoma japonicum cercariae (group A) or with the Schistosoma japonicum cercariae after washed by muddy water (group B). Meanwhile, Ginger oil was besmeared on the abdomen of mice to observe its toxic and side effects to skin. ResultsNone was infected in the Ginger oil group, the infection percentage of the muddy water group was 10%, and the rates of worm reduction were 100% and 88.5%, respectively. There were significant differences among the Ginger oil group, the muddy water group and the conorol group （P＜0.001）. In addition, no obvious injury was observed on the skin of mice when treated with Ginger oil. ConclusionGinger oil has significant protective effect against infection caused by Schistosoma japonicum cercariae with little toxic and side effect. 　　Key words：Ginger oil; Schistosoma japonicum; Cercariae; Mice 　　生姜是姜科姜属植物姜Zingiber offzcinale Rosc 的根茎，生姜油是从姜根茎中提取出来的挥发性油分，具有浓郁的芳香气味,主要用于食品及饮料的加香调味,也是药用原料［1，2］。我们已报道生姜油对日本血吸虫尾蚴有较好的杀灭效果［3］。为了进一步了解生姜油的有关作用，本实验对生姜油预防日本血吸虫尾蚴感染的效果以及对皮肤的毒副作用进行了研究。 　　1 材料 　　生姜油由芜湖杉杉生物技术有限公司提供，采用超临界二氧化碳萃取，为棕红色油状液体。生姜油相对密度（20/200C）0.925，折光系数（200C）1.499。10% 水合氯醛，天津市福晨化学试剂厂出品。尾蚴，由江苏省血吸虫病防治研究所提供阳性钉螺，实验当日逸放日本血吸虫尾蚴。小鼠，昆明种小鼠,3月龄，鼠重20～24 g，雌雄各半，由本学院动物实验中心提供。 　　2 方法 　　2.1