红景天超微粉对乙醇所致小鼠急性肝损伤保护作用.docVIP

红景天超微粉对乙醇所致小鼠急性肝损伤保护作用 　 作者：吕惠子 朴光春 李镐 黄顺福 【摘要】 　　目的探讨红景天超微粉和红景天乙醇提取物对小鼠急性肝损伤的保护作用，同时比较红景天超微粉和红景天乙醇提取物在相同生药剂量下肝保护作用的差异及量效关系。方法分别以红景天超微粉物和乙醇提取物对以酒精制成急性肝损伤模型应用，测定小鼠血清中ALT，AST 含量，肝脏匀浆中MDA含量，SOD的活性，同时做肝组织切片。结果红景天超微粉物和乙醇提取物明显降低因酒精所致的急性肝损伤小鼠血清中 ALT，AST含量，肝匀浆中MDA含量降低和SOD的活性的升高比模型组有显著性差异（Plt;0.01）。结论 红景天超微粉对酒精所致急性肝损伤均有保护作用，且红景天超微粉作用优于乙醇提取物。 【关键词】 红景天超微粉 肝损伤 　　Abstract：Objective To study the protective effect of the ultra fine powder and the ethanol extract of Rhodiola sachalimemsis A.Bor. against acute liver injury in mice，and compare the protective effect and dose-effect relationship between equal dose of them.MethodsThe mice were perfused with ultra fine powder and ethanol extract of Rhodiola sachalimemsis A.Bor. respectively. The mouse model of acute hapatic injury was induced by ethanol and the serum levels of ALT and AST and the content of MDA，activity of SOD in liver of the mice were measured, and tissue slice of liver was made.ResultsThe groups of ultra fine powder and ethanol extract of the Rhodiola sachalimemsis A.Bor. significantly reduced the levels of serum ALT and AST which were induced by ethanol, and the content of MDA, activity of SOD in liver were significant different compared with the model groups (Plt;0.01). ConclusionThe ultra fine powder has a protective effect against hapatic injury, and it was superior to ethanol extract. 　　Key words：Ultra fine powder of Rhodiola sachalimemsis A.Bor. Liver injury 　　高山红景天Rhodiola sachalimemsis A.Bor.是景天科红景天属多年生草本植物。红景天全草都可入药，民间常用于清肺止咳、止血、跌打损伤、烧烫伤、阳痿及糖尿病等病症。近几年来的研究表明红景天苷对小鼠实验性肝损伤有很好的保护作用［4］。但用红景天超微粉对小鼠实验性肝损伤的保护研究尚未见报道。本实验用红景天的超微粉对急性肝损伤的保护作用进行了探讨，同时与乙醇提取物进行了比较研究。 　　1 器材与方法 　　1.1 材料 　　高山红景天购于吉林省安图县药材市场，由延边大学药学院生药学教研部刘永镇教授鉴定为高山红景天Rhodiola sachalimemsis A.Bor.。1kg红景天干燥根用70%乙醇回流提取3次，1h/次，合并3次滤液，减压浓缩得乙醇提取物180 g； 红景天干燥根由四平高斯达纳米材料设备有限公司粉碎，得300 nm,500～1000 nm,1～5 μm 3个粒度的超微粉物。 　　1.2 试剂与仪器 　　55度白酒（北京顺通三星酒业有限公司）， 护肝宁片（吉林省通化天立药业有限公司），ALT，AST试剂盒为北化康泰临床试剂有限公司产品，SOD，MDA试剂盒、考马斯亮兰试剂盒为南京建成生物工程研究所产品。使用的仪器有生化分析仪（SPOTCHEME2，SP4430），DY89-1