缺血性卒中急性期患者红细胞调控白细胞介素 - 18 新实验体系研.docVIP

缺血性卒中急性期患者红细胞调控白细胞介素 - 18 新实验体系研 　【摘要】 目的：采用血液免疫反应路线图实验体系探讨缺血性卒中急性期患者红细胞调控白细胞分泌 IL-18 的能力。方法：将缺血性卒中急性期患者组分为 2 组即大、中小梗死组。将癌细胞（S180）悬液 0.2 mL（10×106/mL）或生理盐水 0.2 mL 加至枸橼酸抗凝全血细胞悬液（或白细胞悬液）0.2 mL 和血浆 0.3 mL 中，37 ℃水浴 1 h，酶联免疫法测定反应 液中 IL-18 含量。结果：大梗死组 IL-18 的含量明显高于中小梗死组和对照组（P lt; 0.01）。中小梗死组无抗原激活组（0.32±0.34）高于抗原激活组（-0.41±0.74）和对照组无抗原激活组（-0.20±0.50）红细胞对白细胞分泌 IL-18 的调控率（P lt; 0.05）。结论：动脉粥样硬化阶段就已经存在红细胞免疫功能的下降，梗塞时更为明显。红细胞对白细胞分 泌 IL-18 具有吸附下调作用，疾病严重时处于失调控阶段。 【关键词】 癌；缺血性卒中，急性期；变态反应和免疫学；红细胞；白细胞介素-18 〔Abstract〕 Objective To approach the capacity of erythrocyte regulating IL-18 in patients with acute ischemic stroke (AIS) by a new experimental system of hemaimmune. Methods Patients with AIS were divided into two groups of the large, the midst and small by the volume of infarction and made group comparison. 0. 2 mL suspension of cancer cells (S180:10 ×106/mL) or NS were added into 0.2 mL anticoagulant suspension of whole blood cells or leukocytes and 0.3 mL plasma, then incubated for 1 h at 37℃. The content of IL-18 was determined by ELISA. Results The content of IL-18 in the large group was much higher than those of the others. The regulative capacity of erythtocyte to IL-18 with cancer cells was lower than without in the midst and small group, and also was lower than that of the control group with cancer cells. Conclusion When in the period of atherosclerotic, the immunity erythrocyte had been declined. And it declined more when in AIS. It could absorb IL-18, while it was in imbalance when the disease was serious. 〔Key Words〕 Carcinoma; Ischemic stroke; Acute; Allergy and immunology; Erythrocytes; Interleukin-18 IL-18 作为一种新近发现的促炎细胞因子，机体的众多器官、组织和细胞都可检测到 IL-I8 mRNA 的存在，外周血单核细胞经内毒素（LPS）刺激也有表达。在神经系统中，小胶质细胞[1]、星型胶质细胞[2]、神经元和室管膜细胞[3]也可产生。IL-18 能够有力地激活 T 细胞和巨噬细胞，产生一系列细胞因子。研究表明，IL-18 参与动脉粥样硬化和缺血性脑卒中的病理过程。 本实验采用“血液免疫反应路线图”[4]理论构建的自然与分离的实验体系，测定缺血性卒中急性期红细胞调控白细胞分泌 IL-18 的水平，证实癌细胞可以激活白细胞分泌 IL-18，红细胞可以吸附白细胞分泌的 IL-18，同时也证明了缺血性卒中急性期红细胞免疫功能低下，对白细胞分泌 IL-18 调控的能力处于失平衡状态。 1 材料与方法 1.1 研究对象 缺血性