原创力文档antitumor activity against murine lymphoma l5178y model of proteins from cacao (theobroma cacao l.) seeds in relation with in vitro antioxidant activity抗肿瘤活性对小鼠淋巴瘤l5178y模型从可可一种(可可树的东西可可l .)种子的蛋白质在体外抗氧化活性的关系.pdfVIP
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Preza et al. BMC Complementary and Alternative Medicine 2010, 10:61
/1472-6882/10/61
RESEARCH ARTICLE Open Access
Antitumor activity against murine lymphoma
L5178Y model of proteins from cacao
(Theobroma cacao L.) seeds in relation with
in vitro antioxidant activity
Ana M Preza1†, María E Jaramillo1†, Ana M Puebla2†, Juan C Mateos3†, Rodolfo Hernández3†, Eugenia Lugo3*
Abstract
Background: Recently, proteins and peptides have become an added value to foodstuffs due to new knowledge
about its structural analyses as related to antioxidant and anticancer activity. Our goal was to evaluate if protein
fractions from cacao seeds show antitumor activity on lymphoma murine L5178Y model. The antioxidant activity of
these fractions was also evaluated with the aim of finding a correlation with the antitumor activity.
Methods: Differential extraction of proteins from unfermented and semi-fermented-dry cacao seeds was
performed and characterized by SDSand FPLC size-exclusion chromatography. Antitumor activity was
evaluated against murine lymphoma L5178Y in BALB/c mice (6 × 104 cells i.p.), with a treatment oral dose of 25
mg/kg/day of each protein fraction, over a period of 15 days. Antioxidant activity was evaluated by the ABTS+ and
ORAC-FL assays.
Results: Albumin, globulin and glutelin fractions from both cacao seed type were obtained by differential solubility
extraction. Glutelins were the predominant fraction. In the albumin fraction, polypeptides of 42.3 and 8.5 kDa were
found in native conditions, presumably in the form of two peptide chains of 21.5 kDa each one. The globulin
fraction presented polypeptides of 86 and 57 kDa in unfermented cacao seed that produced the specific-cacao
aroma precursors, and after fermentation the polypeptides were of 45 and 39 kDa. The glutelin fraction presented
proteins 200 kDa and g
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