association of hpa axis-related genetic variation with stress reactivity and aggressive behaviour in pigs协会hpa axis-related遗传变异与压力反应性和侵略性的行为在猪身上.pdfVIP

Muráni et al. BMC Genetics 2010, 11:74 /1471-2156/11/74 RESEARCH ARTICLE Open Access Association of HPA axis-related genetic variation with stress reactivity and aggressive behaviour in pigs 1 2 3 3 4 5 6 Eduard Muráni , Siriluck Ponsuksili , Richard B D’Eath , Simon P Turner , Esra Kurt , Gary Evans , Ludger Thölking , Ronald Klont6 7 7 1* , Aline Foury , Pierre Mormède , Klaus Wimmers Abstract Background: Stress, elicited for example by aggressive interactions, has negative effects on various biological functions including immune defence, reproduction, growth, and, in livestock, on product quality. Stress response and aggressiveness are mutually interrelated and show large interindividual variation, partly attributable to genetic factors. In the pig little is known about the molecular-genetic background of the variation in stress responsiveness and aggressiveness. To identify candidate genes we analyzed association of DNA markers in each of ten genes (CRH g.233CT, CRHR1 c.*866_867insA, CRHBP c.51GA, POMC c.293_298del, MC2R c.306TG, NR3C1 c.*2122AG, AVP c.207AG, AVPR1B c.1084AG, UCN g.1329TC, CRHR2 c.*13TC) related to the hypothalamic-pituitary- adrenocortical (HPA) axis, one of the main stress-response systems, with various stress- and aggression-related parameters at slaughter. These parameters were: physiological measures of the stress response (plasma concentrations of cortisol, creatine kinase, glucose, and lactate), adrenal weight (which is a parameter reflecting activity of the central branch of the HPA axis over time) and aggressive behaviour (measured by means of lesion scoring) in the context of psychosocial