association of il1b -511c-31t haplotype and helicobacter pylori vaca genotypes with gastric ulcer and chronic gastritis协会-511年il1b c-31t单体型和幽门螺杆菌vaca基因型胃溃疡、慢性胃炎.pdfVIP

Martínez-Carrillo et al. BMC Gastroenterology 2010, 10:126 /1471-230X/10/126 RESEARCH ARTICLE Open Access Association of IL1B -511C/-31T haplotype and Helicobacter pylori vacA genotypes with gastric ulcer and chronic gastritis 1 2 3 1 Dinorah N Martínez-Carrillo , Elvira Garza-González , Reyes Betancourt-Linares , Trinidad Mónico-Manzano , Cuauhtémoc Antúnez-Rivera1 1 4 5 , Adolfo Román-Román , Eugenia Flores-Alfaro , Berenice Illades-Aguiar , Gloria Fernández-Tilapa1* Abstract Background: The association between proinflammatory cytokine gene polymorphisms and gastric diseases related to Helicobacter pylori varies by population and geographic area. Our objective was to determine if the IL-1B -511 TC and -31 CT polymorphisms and H. pylori vacA genotypes are associated with risk of chronic gastritis and gastric ulcer in a Mexican population. Methods: We conducted endoscopic studies in 128 patients with symptoms of dyspepsia. We took two biopsies from the body, antrum, or ulcer edge from each patient, and classified our histopathological findings according to the Sydney System. H. pylori infection and vacA genotyping were accomplished via PCR from total DNA of the gastric biopsies. We confirmed the presence of anti-H. pylori serum IgG and IgM in 102 control subjects. In both case subjects and control subjects, the IL-1B -511 TC polymorphism was genotyped by PCR-RFLPs and the IL-1B -31 CT polymorphism was genotyped by pyrosequencing. Results: Sixty-two point seven (62.7%) of the 102 control subjects were H. pylori-seropositive. Among the case subjects, 100 were diag