美登木根的显微结构和其内生真菌的分布.pdfVIP

植物学通报 2006, 23 (4): 368~373 Chinese Bulletin of Botany . . 实验简报 美登木根的显微结构及其内生真菌的分布 2 1* 2 2 谭小明 郭顺星 周雅琴 缪剑华 ( 1 中国医学科学院中国协和医科大学药用植物研究所药用植物菌根研究室 北京 100094) (2 广西药用植物园 南宁 530023) 摘要 本文采用石蜡永久制片和光学显微摄像的方法对美登木(Maytenus confertiflorus)根的显微结构及 其内生真菌的分布进行了研究。结果表明, 美登木根的次生结构由周皮和维管组织构成; 周皮由木栓层、 木栓形成层和栓内层组成, 其中木栓层由5～6 列长形细胞组成; 维管组织中次生韧皮部所占根径的比例 达46%, 其薄壁细胞中内含物较丰富, 次生木质部中分布有导管和木射线及少量木薄壁组织; 在美登木木 栓层和次生韧皮部中分布有菌丝片段、膨大的菌丝、菌丝团及分生孢子; 内生真菌只在一定区域的皮层 和次生韧皮部细胞中分布。 关键词 根的显微结构, 美登木, 内生真菌, 石蜡切片 Microstructure and Endophytic Fungus Distribution of Maytenus confertiflorus Root 2 1* 2 2 Xiaoming Tan , Shunxing Guo , Yaqin Zhou , Jianhua Miu (1 Mycorrhizal Department , Institute of Medicinal Plant Development , CAMS and PUMC , Beijing 100094) (2 Guangxi Botanical Garden of Medicinal Plant, Nanning 530023 ) Abstract The microstructure and distribution of endophytic fungi in Maytenus confertiflorus root were studied by permanent paraffin sectioning and microscope photography. The secondary struc- ture of the root consisted of periderm and vascular cylinders. Periderm was composed of cork, cork cambium, and phelloderm. The cork consisted of five or six layers of long cells. The secondary phloem occupied 46% of the diameter of the root and the parenchymas cells had abundant inclusions. The secondary xylem consisted of vessels, and xylary radial and some xylary parenchyma cells. Some segments of hypha, swelled hypha, pelotons and conidiophore were in colonies in cork and second- ary phloem. Endophytic fungi existed in the local cells of cork and secondary phloem in the root. Key words microstructure of root, Maytenus confertiflorus, endophytic fungi, permanent paraffin- cut section 美登木(Mayt