targeted disruption of py235ebp-1 invasion of erythrocytes by plasmodium yoelii using an alternative py235 erythrocyte binding protein针对中断py235ebp-1由疟原虫入侵红细胞yoelii使用另一种py235红细胞结合蛋白.pdfVIP

Targeted Disruption of py235ebp-1: Invasion of Erythrocytes by Plasmodium yoelii Using an Alternative Py235 Erythrocyte Binding Protein 1 2 3 4 1 Solabomi A. Ogun *, Rita Tewari , Thomas D. Otto , Steven A. Howell , Ellen Knuepfer , Deirdre A. 1 4 3,5 1 Cunningham , Zhengyao Xu , Arnab Pain , Anthony A. Holder * 1 Division of Parasitology, MRC National Institute for Medical Research, London, United Kingdom, 2 Institute of Genetics, University of Nottingham, Nottingham, United Kingdom, 3 Parasite Genomics, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom, 4 Protein Structure, MRC National Institute for Medical Research, London, United Kingdom, 5 Computational Bioscience Research Center, Chemical and Life Sciences and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia Abstract Plasmodium yoelii YM asexual blood stage parasites express multiple members of the py235 gene family, part of the super- family of genes including those coding for Plasmodium vivax reticulocyte binding proteins and Plasmodium falciparum RH proteins. We previously identified a Py235 erythrocyte binding protein (Py235EBP-1, encoded by the PY01365 gene) that is recognized by protective mAb 25.77. Proteins recognized by a second protective mAb 25.37 have been identified by mass spectrometry and are encoded by two genes, PY01185 and PY05995/PY03534. We deleted the PY01365 gene and examined the phenotype. The expression of the members of the py235 family in both the WT and gene deletion parasites was measured by quantitative RT-PCR and RNA-Seq. py235ebp