the 3′ untranslated regions of influenza genomic sequences are 5′ppp-independent ligands for rig-i流感病毒基因组序列的3u2032未翻译区5u2032ppp-independent rig - i的配体.pdfVIP

The 3 9 Untranslated Regions of Influenza Genomic Sequences Are 59PPP-Independent Ligands for RIG-I 1. 1. 2. 1 1 William G. Davis , J. Bradford Bowzard , Suresh D. Sharma , Mayim E. Wiens , Priya Ranjan , 1 3 3 1 1 Shivaprakash Gangappa , Olga Stuchlik , Jan Pohl , Ruben O. Donis , Jacqueline M. Katz , 2 4 1 Craig E. Cameron , Takashi Fujita *, Suryaprakash Sambhara * 1 Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 2 Pennsylvania State University, University Park, Pennsylvania, United States of America, 3 Division of Scientific Resources, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America, 4 Kyoto University, Kyoto, Japan Abstract Retinoic acid inducible gene-I (RIG-I) is a key regulator of antiviral immunity. RIG-I is generally thought to be activated by ssRNA species containing a 59-triphosphate (PPP) group or by unphosphorylated dsRNA up to ,300 bp in length. However, it is not yet clear how changes in the length, nucleotide sequence, secondary structure, and 59 end modification affect the abilities of these ligands to bind and activate RIG-I. To further investigate these parameters in the context of naturally occurring ligands, we examined RNA sequences derived from the 59 and 39 untranslated regions (UTR) of the influenza virus NS1 gene segment. As expected, RIG-I-depend