curing of plasmid pxo1 from bacillus anthracis using plasmid incompatibility治疗炭疽杆菌的质粒pxo1用质粒不相容.pdfVIP

Curing of Plasmid pXO1 from Bacillus anthracis Using Plasmid Incompatibility Xiankai Liu., Dongshu Wang., Huagui Wang, Erling Feng, Li Zhu, Hengliang Wang* State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, Beijing, China Abstract The large plasmid pXO1 encoding the anthrax toxin is important for the virulence of Bacillus anthracis. It is essential to cure pXO1 from B. anthracis to evaluate its role in the pathogenesis of anthrax infection. Because conventional methods for curing plasmids (e.g., curing agents or grow