pia水稻悬浮细胞系的建立.pdfVIP

生 物 工 程 学 报 Chin J Biotech 2009, March 25; 25(3): 424-427 Chinese Journal of Biotechnology ISSN 1000-3061 cjb@ © 2009 Institute of Microbiology, CAS CSM, All rights reserved 农业生物技术 PiA 1, 2* 1* 1 1 1 1 1, 2 张红宇 , 杨富云 , 高梅 , 徐培洲 , 张全芳 , 徐建第 , 吴先军 1 , 611130 2 , 625014 : 本研究以抗稻瘟病的粳稻PiA 开花后 12~15 d 的幼胚为材料, 将诱导 10~15 d 的愈伤不经固体培养基继代, 直 接转到AA 液体培养基上, 在较短时间内成功建立起了优良的水稻悬浮细胞系; 并测定了该悬浮细胞系不同时期的生长 特性和分化情况, 结果表明悬浮培养适宜的继代天数是 7~10 d; 培养 30~120 d 的细胞分化能力和植株再生能力较好, 细胞分化率和成苗率分别为57.1%和20%, 为进一步利用悬浮细胞进行遗传转化和原生质体分离奠定了基础。 : 水稻, 悬浮细胞系, 细胞分化, PiA Rapid establishment of suspension cell lines in japonica rice 1, 2* 1* 1 1 1 1 Hongyu Zhang , Fuyun Yang , Mei Gao , Peizhou Xu , Quanfang Zhang , Jiandi Xu , and Xianjun Wu 1, 2 1 Rice Research Institute of Sichuan Agricultural University, Wenjiang 611130, China 2 Key Laboratory of Crop Gene Resources and Improvement, Ministry of Education , Sichuan Agricultural University, Ya’an 625014, China Abstract: With Jingkang No.5 (PiA), calli of the PiA induced for 10−15 days were transferred into amino acid liquid culture medium, to establish excellent rice suspension cell lines successfully in a relative short time. The growth characteristics and differentiation conditions of suspension cells were measured at different phases. Results revealed that the optimal subculture time was 7−10 days, and the cells cultured for