Bio-Rad荧光定量PCR应用指南(完整版).pdf

References Mathews DH et al., Expanded sequence dependence of thermodynamic parameters provides robust pre- diction of RNA secondary structure, J Mol Biol 288, 910–940 (1999) Rozen S and Skaletsky HJ, Primer3 on the WWW for general users and for biologist programmers, pp 365–386 in Bioinformatics Methods and Protocols: Methods in Molecular Biology (Krawetz S and Misener S, eds), Humana Press, Totowa, NJ (2000); source code available at /primer3/ Zuker M et al., Algorithms and thermodynamics for RNA secondary structure prediction: a practical guide, pp 11–43 in RNA Biochemistry and Biotechnology (Barciszewski J and Clark BFC, eds), Kluwer Academic Publishers, Boston (1999) Legal Notices Amplifluor and UniPrimer are trademarks of Millipore Corporation. AmpliTaq, AmpliTaq Gold, and TaqMan are trademarks of Roche Molecular Systems, Inc. BD QZyme is a trademark of Becton, Dickinson and Company. Beacon Designer is a trademark of PREMIER Biosoft International. BHQ and Black Hole Quencher are trademarks of Biosearch Technologies, Inc. Cy is a trademark of GE Healthcare. DNeasy is a trademark of Qiagen GmbH Corporation. DyNAzyme is a trademark of Finnzymes Oy. Eclipse is a trade- mark of Epoch Biosciences, Inc. Excel is a trademark of Microsoft Corporation. Hoechst is a trademark of Hoechst Aktiengesellschaft. LUX is a trademark of Invitrogen Corporation. Oligo is a trademark of Molecular Biology Insights, Inc. PicoGreen, SYBR, and Texas Red are trademarks of Molecular Probes, Inc. Roundup Ready is a trademark of Monsanto Company. Scorpions is a trademark of DxS Limited. VIC is a trademark of Applera Corporation. Molecular beacons and their use are licensed under patents owned by The Public Health Research Institute of the City of New York, Inc. Primer3 includes software developed by the Whitehead Institute for Biomedical Research. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell reagents containing SYBR Green I for use in rea