Wntβ-catenin通路在小鼠肾发育中的作用及差异表达基因.pdfVIP

CHINESE OFANATOMYV01．36No．52013 JOURNAL 周静袁伟杰△ (上海交通大学附属第一人民医院肾内科，上海200080) 摘要 目的：探讨Wnt／p-catenin通路在小鼠肾发育中的作用及差异表达基因。方法：采用基因芯片技术筛选小鼠肾发育 过程中的差异表达基因；应用实时定量RT-PCR和免疫印迹观察不同胎龄小鼠。肾组织中的pcateninmRNA和活化的p 与定量。结果：13-eatenin E14．5 d达到高峰，后逐渐下降。在出生后1d出现小高峰后，逐渐下降并维持在低水平。免疫荧光显示，在小鼠肾发生发 间和空间表达在肾发育中发挥重要的作用。 关键词 Wnt／13-catenin信号通路；肾；发育 of signalingpathwaykidneydevelopment+ ExpressionWnt／Pcatenin during Zhou Wenie△ Jing，Yuan (DepartmentofNephrology，ShanghaiFi￡People’SHospital，ShanghaiJiaotongUniversity，Shanghai Abstract the of mouse and Objective：Tostudyexpression signalingpathwayduring kidneydevelopmentgene Wnt／p-catenin wereusedtoscreendifferential RT-PCRandWestern wereusedtosemi— chip expressedgenes．Methods：Real—time blotting the and measure of mRNA ofmouse quantitativelyexpression13-catenin proteinduringdevelopmentkidney． active』3-catenin Immunohistochemicalwasusedtoexaminethe10cationofactive atdifferentof and staining protein stagesembryonic pcatenin mRNAwas atdifferenttime of postnatalkidney．Results：Active expresseddifferently p-catenin pointskidneydevelopment． Andtheactive reachedatE14．5dandthen decreaseduntilthefirst afterbirth，andthen protein peak gradually day p-catenin decreased andmaintainedinlOWlevel2weeksafterbirth．Immunohistochemicalsho