同源四倍体水稻胚乳发育极核融合和胚乳细胞化.pdfVIP

Rice 281 Sci)，2004，18(4)：281～289 中国水稻科学(ChineseJ 同源四倍体水稻胚乳发育：极核融合和胚乳细胞化 王 兰1 刘向东1，” 卢永根1 冯九焕1 徐雪宾1 徐是雄2 (1华南农业大学广东省植物分子育种重点实验室，广东广州510642；2香港大学植物系，香港薄扶林道；”通讯联系人) in Rice：TheFusionofPolarNucleiand Autotetraploid EndospermDevelopment theFormationof CellWall Endosperm WANGLanl，LIu Jiu—huanl，XuXue-binl，Xu Xiang—don91一，LuYong—genl，FENG Shi—xiong(S．Y．Zee)2 Molecular (1 Provincial Plant Breeding，SouthChina 510642，China： Guangdong KeyLaboratoryof AgriculturalUniversity，Guangzhou author) 2DepartmentofBotany，theUniversityofHongKong，PokfulamRoad，HongKong，China；’Corresponding thelaser confocal and 7022histeresin kit)semi-thin Abstract：Byusing scanning microscopyplastic(Leica embedding fusionof nucleithe technique，comparativestudiesonthe polar and formationofthewallof cellsinau— sectioning endosperm and ricewerecarriedOUt．Twoofovarieswereobservedinthe totetraploiddiploid types autotetraploid．0netypedisplayed normal of nucleifusionandfurther offreenucleisimilartOthoseinovariesinthe oth— processpolar development diploid．The ershowedkindsofabnormal of many developmentpolarnuclei，includingnon-fertilization，singlefertilization，polarnuclei fusionwithoutmitosisand of nuclei．The of cellwallin non-synchronizationendosperm developmentendosperm autotetraploid wasthesameasthatin wall of sacnearth