市售川乌各品种中总黄酮的含量比较Δ.pdfVIP

市售川乌各品种中总黄酮的含量比较Δ ＊ # 郑玲利 ，袁明勇 ，朱盛林，赵 霞（成都医学院第一附属医院药剂科，成都 610500） 中图分类号 R284.1；R927.2 文献标志码 A 文章编号 1001-0408（2013）31-2942-03 DOI 10.6039/j.issn. 1001-0408.2013.31.22 摘 要 目的：测定市售川乌不同品种中总黄酮的含量并比较其差异。方法：利用超声波提取法，在温度55 ℃、时间40min 、以 70％乙醇为提取溶剂、物料比为1∶20（m/ V）的条件下提取10个样品中的黄酮类化合物；以芦丁为对照品，在510nm 波长处，采用 紫外分光光度法测定总黄酮的含量。结果：芦丁的质量浓度在0～0.0988mg/ml 范围内与其吸光度呈良好的线性关系（r ＝ 0.9998）；精密度、稳定性、重复性试验的RSD 均＜2％；平均加样回收率为97.93％，RSD ＝1.55％（n ＝6）。10个市售川乌样品中， 总黄酮质量分数最高的是8号，其质量分数极显著高于10、4、6、7、2、1、9、3号，显著高于5号；3号川乌的总黄酮质量分数最低，但 与7、2、1、9号川乌比较差异无统计学意义。结论：市售不同品种川乌中总黄酮的质量分数存在明显差异，临床应注意甄别使用。 关键词 川乌；黄酮类化合物；市售品；紫外分光光度法 Comparison of the Flavonoids Content from Marketable Aconitum carmichaelii Products ZHENG Ling-li ，YUAN Ming-yong ，ZHU Sheng-lin ，ZHAO Xia （Dept. of Pharmacy ，The First Affiliated Hospi- tal of Chengdu Medical College ，Chengdu 610500，China ） ABSTRACT OBJECTIVE ：To determine and compare the contents of flavonoids from different types of marketable Aconitum car- michaelii. METHODS ：Using ultrasonic extraction ，the flavonoids compounds were extracted from 10samples at 55 ℃for 40 min with the material ratio of 1∶20 （m/ V）using 70％ethanol as solvent. The content of flavonoids was determined by UV spectropho- tometry at 510nm ，using rutin as substance control. RESULTS ：The linear range of rutin were 0-0.098 8mg/ml （r ＝0.999 8）with an average recovery of 97.93％（RSD ＝1.55％，n ＝6）. RSDs of precision ，stability and reproducibility tests were all lower than 2％. Among 10types of marketable A. carmichaelii ，the content of flavonoids in No. 8 sample was the highest ，which was very sig- nificantly higher than in No. 10，No.4，No. 6，No.7，No.2，No. 1，No.9 and No.3 samples ，and significantly higher than in No.5 samples ；the content flavonoids in No. 3 sample was the lowest ，which was not significantly different from No. 7，No.2，No. 1and No.9 samples. CONCLUSIONS ：The