TRPC3参与缺氧诱导人脐静脉血管内皮细胞凋亡-第三军医大学学报.DOC

TRPC3参与电磁辐射致海马神经元凋亡 文莉莉1，米永杰2，李茂全（614200四川峨眉成都军区峨眉疗养院610083成都成都医学院人体解剖与组织胚胎学教研室成都医学院公共卫生系）瞬时受体电位通道蛋白3 （transient receptor potential channel 3TRPC3）诱导凋亡。方法以 mW/cm2电磁辐照培养Wistar大鼠海马神经元20 min，以未接受电磁波辐照的神经元为对照组，将TRPC3-siRNA和对照寡核苷酸转染神经元后分别设立为辐照+TRPC3-siRNA干扰组和辐照＋TRPC3假干扰组。采用实时定量RT-PCR法及法检测TRPC3 mRNA和蛋白水平的表达后用CCK8各组细胞存活率，Annexin VFITC试剂盒染色细胞凋亡。结果 中TRPC3 mRNA和蛋白表达，细胞存活率为.8%，与对照组有显著差异（P0.05）；+TRPC3干扰组细胞存活率为.3%，凋亡率为9.2%， 与组有显著差异（P0.05）＋TRPC3假干扰组与组无明显差异（P＞0.05）。结论 TRPC3凋亡。lectromagnetic irradiation-induced apoptosis of hippocampal neurons through TRPC3 Wen Lili 1，Mi Yongjie2，Chen Chunhai3， Li Maoquan4（1. Department of Medical Administration, Emei Sanatorium of Chengdu Military Region，Sichuan 614200，China； 2. Department of Human Anatomy，Histology and Embryology Chengdu Medical College，Chengdu， Sichuan 610083Department of Occupational Health, Third Military Medical University，Chongqing 400038，China；4. Department of Public Health，Chengdu Medical College，Chengdu， Sichuan 610083Objective To explore whether TRPC3 plays a role on apoptosis of hippocampal neurons induced by electromagnetic irradiation. Methods Cultured hippocampal neurons of Wistar rats were exposed to 65 mW/cm2 electromagnetic wave for 20 min. Using real-time RT-PCR and western blotting， TRPC3 mRNA and protein expression were detected. The TRPC3siRNA or control-siRNA was transfected into neurons. The viability of the neurons was determined by CCK8 assay， and the apoptosis were measured by flow cytometry after AnnexinV-FITC staining. Results Electromagnetic irradiation markedly increased TRPC3 mRNA, protein expression and [Ca2+]i in cultured hippocampal neurons. The viability of hippocampal neurons （%） was decreased and the apoptosis rate was increased （30.2%） compared with the control cells （P 0.05）. TRPC3siRNA significantly increased the survival rate （%） and decreased the apoptosis rate （9.2%）and partially suppressed [Ca2+]i of the irradiation-treated cells（P 0.05），TRPC3 false-siRNA treatment did not have thes