支气管哮喘豚鼠气道上皮细胞信号转导子与转录活化因子深圳大学.pdfVIP

306 2004 527 5 Chin J Tuberc Respir Dis, May 2004, Vol. 27, o.5 1 李国平 熊瑛 刘志刚 钟南山 1 (STAT-1) ( ) STAT-1 , STAT-1 48 (A ,8 )(B ) ( C, 8) , B B B B ( 8) BC 10%(OVA) 1 2 3 4 ,0 h24 h72 h5 d STAT-1 1(ICAM-1) , STAT-1 , (BALF) , ( IF -) A STAT-1 ICAM-1 13721 8, (B1~ 4) (0 h 24 h72 h5 d) STAT-1 ICMA-157 9136 1495 2167 30;75 10166 17113 1487 21B1~ 4A ( P 001) ; STAT-1 ( r= 0652, P 001) ; STAT-1 ICMA-1 ( r= 0556, P 0001) , I F- STAT-1 ( r = - 0499, P 005) STAT-1, , ; ; ; 1; 1 The role of signal transducer and activator of transcription-1 in the regulation of allergic airway * inflammation in a guinea pig asthma model LI Guo-p ing , XIONG Ying, LIU Zhi-gang, ZHONG Nan- * shan. Dep artment of Resp iratory Diseases, ff iliated Hospital of Luzhou Medical College, Luzhou 646000, China Abstract Objective To study the expression of signal transducer and activator of transcription-1(STAT- 1) , and to investigate the effect and mechanism of STAT-1 on airway inflammation Methods Forty-eight guinea pigs were divided randomly into control group ( group A, n = 8) , asthma group(group B) and prevention group ( group C, n= 8)The guinea pigs in group B were subdivided randomly into groups B , B , B and B (each n 1 2 3 4 = 8)Asthma guinea pig models in group B and group C were set up by intraperitoneal injection and nebulization aspiration of ovalbumin( OVA) Expression levels of epithelial STAT-1 and intercellular adhesion molecule-1 ( ICAM-1) were detected by immunohistochemisty, and lung tissue extracts were analyzed for phosphorylation of STAT-1 by western blot Total cell and eosinophil ( EOS) counts were examined, and expression levels of interferon-gamma (