高通量转录组测序技术在植物雄性不育研究中的应用-遗传.pdfVIP

Hereditas (Beijing) 2016 年 8 月, 38(8): 677 ―687 综 述 高通量转录组测序技术在植物雄性不育研究中的应用 刘永明，张玲，邱涛，赵卓凡，曹墨菊 四川农业大学玉米研究所，成都 611130 摘要 : 植物雄性不育是指植物雄蕊发育受阻不能产生正常有功能花粉的现象。植物雄性不育不仅是生殖生理研 究的宝贵材料，也是植物杂种优势利用的重要工具。由于高通量转录组测序技术几乎可以检测细胞内所有 mRNA 及非编码RNA 的信息，已被广泛应用于生命科学研究的各项领域。在植物雄性不育相关研究中，高通 量转录组测序技术在不同物种、不同败育类型中的应用已有报道，这为研究者在转录组水平综合了解植物雄性 不育的分子机制及代谢网络提供了帮助。本文从测序文库构建策略、差异表达基因、非编码RNA 的功能特征 等方面综述了高通量转录组测序在植物雄性不育机理方面的研究进展，并探讨了转录组测序技术在花粉败育机 制解析及育性相关基因定位中的应用价值，以期为植物雄性不育的相关研究提供参考。 关键词 : 植物雄性不育；转录组；转录组测序；非编码RNA Research progress on mechanisms of male sterility in plants based on high-throughput RNA sequencing Yongming Liu, Ling Zhang, Tao Qiu, Zhuofan Zhao, Moju Cao Maize Research Institute, Sichuan Agricultural University, Chengdu 611130, China Abstract : Male sterility is defined as failing to produce functional pollen during stamen development in plants, and it plays a crucial role in plant reproductive research and hybrid seed production in utilization of crop heterosis. High through- put RNA sequencing (RNA-seq) has been used widely in the study of different fields of life science, as it readily detects all the mRNA and non-coding RNA in cells. Recently, RNA-seq has been reported to be applied in different species and kinds of pollen abortion types in plants, which has contributed to the understanding of the molecular mechanism and metabolic networks of male sterility at the transcription level. In this review, we summarize research progress on the mechanisms of male sterility in plants, focusing on RNA-seq analysis encompassing strategies of RNA library construction, differentially expressed genes and functional characteristics of noncoding RNAs involved in stamen abortion. Furthermore, we also dis- cuss application of transcriptome sequencing technology to elucidate pollen abortion mechanisms and map fertility-related genes. We hope to provide references to the study