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Bioresource Technology 100 (2009) 2562–2568 Contents lists available at ScienceDirect Bioresource Technology journal homepage: /locate/biortech Bioethanol, biohydrogen and biogas production from wheat straw in a biorefinery concept Prasad Kaparaju a, María Serrano a, Anne Belinda Thomsen b, Prawit Kongjan a, Irini Angelidaki a,* a Department of Environmental Engineering, Technical University of Denmark, Building 115, DK-2800 Kgs. Lyngby, Denmark b Biosystems Department, RISØ-DTU, Building 301, DK-4000, Roskilde, Denmark a r t i c l e i n f o a b s t r a c t Article history: The production of bioethanol, biohydrogen and biogas from wheat straw was investigated within a bior- Received 29 June 2008 efinery framework. Initially, wheat straw was hydrothermally liberated to a cellulose rich fiber fraction Received in revised form 10 November 2008 and a hemicellulose rich liquid fraction (hydrolysate). Enzymatic hydrolysis and subsequent fermenta- Accepted 11 November 2008 tion of cellulose yielded 0.41 g-ethanol/g-glucose, while dark fermentation of hydrolysate produced Available online 8 January 2009 178.0 ml-H2/g-sugars. The effluents from both bioethanol and biohydrogen processes were further used to produce methane with the yields of 0.324 and 0.381 m3/kg volatile solids (VS)added, respectively. Addi- Keywords: tionally, evaluation of six different wheat straw-to-biofuel production scenari