一株具有抗疟活性的金鸡纳树内生真菌的研究-oalib.pdfVIP

菌物学报 jwxt@ 15 March 2013, 32(2): 309-313 Http:// Mycosystema ISSN1672-6472 CN11-5180/Q © 2013 IMCAS, all rights reserved. ·简报· 一株具有抗疟活性的金鸡纳树内生真菌的研究 1,2* 2 1 1 1 周松林 陈双林 黄风迎 王华 赵焕阁 1 海南医学院热带病重点实验室 海南 海口 571101 2 南京师范大学生命科学学院 江苏 南京 210046 摘 要：为筛选具抗疟疾活性的的内生真菌，采用平板分离法对海南金鸡那树内生真菌进行分离纯化。利用“4 日抑 制疟疾”测试内生真菌发酵液的抗疟活性，采用 TLC、HPLC 等分析技术对内生菌发酵液等成分进行分析。从金鸡 那树叶片分离到 31 种内生真菌，通过筛选找到了 1 株具有抗疟疾活性的产奎宁或奎宁类似物的内生真菌。从金鸡那 树叶片中分离得到 1 株产奎宁的结实串孢霉属内生真菌，为奎宁的资源开发开辟了新途径。 关键词：内生真菌，金鸡那树，抗疟活性 Antimalarial substances of an endophytic fungus isolated from cinchona tree ZHOU Song-Lin1, 2* CHEN Shuang-Lin2 HUANG Feng-Ying1 WANG Hua1 ZHAO Huan-Ge1 1Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou, Hainan 571101, China 2 College of Life Sciences, Nanjing Normal University, Nanjing, Jingsu 210046, China Abstract: Plate method was used to isolate and purify endophytic fungi from Cinchona sp. in Hainan Province. The anti-malaria activity of fermented liquid of the endophytic fungi was tested using “4 day inhibit malaria”, and the components such as quinine or structural analogues were analysed using TLC and HPLC. Of the 31 endophytic species isolated from cinchona tree one strain producing quinine or quinine analogues with antimalarial activity was obtained. Morphological observation indicated that this endophytic fungus could be identified as Hormiscium sp. Key words: endophytic fungi, cinchona tree, antimalarial activity 基金项目：国家自然科学基金（No.；海南省自然科学基金（No. 808157 ） * Corresponding author. E-mail: zhousonglin106@163.com 收稿日期: 2012-03-28