兰属杂交兰绿翡翠组培变异的rapd检测与鉴定-广东农业科学.pdfVIP

广东农业科学 2014 年第 21 期 135 兰属杂 兰绿翡翠组培变异的 RAPD 检测与鉴定 肖文芳 李 佐 尤 毅 陈 明 吕复兵 广东省农科院环境园艺研究所/广东省园林花卉种质创新综合利用重点实验室袁 广东 广州 510640冤 要 从兰属杂交兰绿翡翠组培 体中筛选得到表型突变体 8 株袁与正 植株相比多分蘖且矮化袁部分植株 叶卷曲遥 利用 50 条随机引物对突变体和正 株进行 RAPD 检测袁结果显示袁RAPD 检测对突变体检测有效袁5 条动态 性引物的扩增结果都是正 株比变异株的扩增条带多袁表明这类变异很有可能是染色体缺失引起的遥 另外袁发现突 变体存 个体差异袁并与表型的个体差异不对应遥 关键词 杂交兰曰 RAPD 曰 组培变异曰 分蘖 中图分类号 S685.31;Q319 文献标识码 A 文章编号 1004-874X 2014冤21-0135-05 Detection and identification of high-tillering and dwarf mutants from Cymbidium hybrids 耶Lv Fei Cui 爷 by RAPD XIAO Wen-fang, LI Zuo, YOU Yi, CHEN He-ming, LYU Fu-bing (1.Floric ulture Rese arc h Ins titute , G uangdong A cade my of A gric ultural Sc ie nce s/Guangdong Key Lab of Orname ntal Plant G e rmplas m Innov ation and Utilization, G uangz hou 510640, China) Abstract: Eight high-tillering and dwarf mutants were screened from Cymbidium hy brids 耶Lv Fei Cui 爷, some mutants 爷 leaves were curly. A ll the mutants were compared and analyzed with the control (wild -ty pe Cymbidium hy brids) by RAPD using 50 UBC arbitrary primers. The results showed that RA PD analysis was useful for the mutants, the polymorphism between the mutants and the control was detected by using 5 primers and the mutants showed more poly morphic fragments, w hich suggested that this kind of variation was likely to be caused by lack of chromosome. Further, different incised mutant had different polymorphic fragments with w ide individual variability. Key words: Cymbidium hybrids; RAPD; somaclonal variation 曰 tiller 兰科植物是开花植物中最大的科袁有 750 多个属袁 化生产主要通过组织培养生产分生苗和分株两条