浸提法提取秦巴山区枇杷叶中总黄酮含量.pdfVIP

试验报告与理论研究 饮 料 工 业 浸提法提取秦巴山区枇杷叶中总黄酮含量 赵红红，李 波，刘 欣 （ 723001 陕西理工学院化学与环境科学学院，陕西 汉中 ） 摘 要：黄酮类化合物广泛存在于自然界，在医药、酒类、食品和饮料等方面有着广泛应用。本实验通过浸提法对秦 巴山区枇杷叶中黄酮类化合物进行研究，考察提取剂、料液比、提取剂浓度和浸泡时间对总黄酮浸出率的影响，用单 因素实验确定了影响总黄酮的主要因素，通过正交试验确定枇杷叶中总黄酮的最佳提取工艺为：以甲醇为提取剂， 料液比为 1 ∶25 ，提取剂浓度为60%，浸泡时间30min，在此条件下测得总黄酮提取率为8.106%。 关键词：枇杷叶；黄酮类化合物；甲醇；提取工艺；浸提法 Total Extraction of Flavonoid in Loquat Leaves in Qinba Mountain ZHAO Hong-hong ，LI Bo ，LIU Xin College of Chemistry and Environmental Science Shaanxi Univer sity of Technology Hanzhong 723001 China （ ， ， ， ， ） Flavonoids compounds are widespread in nature and are applied in medicine wine food and beverage. In Abstract ： ， ， this paper flavonoids compounds in loquat leave in Qinba Mountain were studied through extraction and effects of ， ， extraction agent ，solid-liquid ratio，concentration of extraction agent and extraction time on extraction rate were determined. The main factors influencing the flavones were determined by single factor experiment. The optimal extraction process was determined by orthogonal experiments. With methyl alcohol as extraction agent the result was ， as follows: the solid-liquid ratio is 1 ∶25 extraction agent concentration is 60% time is 30mins. Extraction rate of ；