假眼小绿叶蝉对茶梢挥发物的行为反应-应用昆虫学报.doc

假眼小绿叶蝉对茶梢挥发物的行为反应* 王梦馨(( 李辉仙 武文竹 孙海潮 石松平丁 源 曹春晖 韩宝瑜((( （中国计量大学生命科学学院，浙江省生物计量及检验检疫技术重点实验室，杭州 310018） 摘 要 【目的】 研究假眼小绿叶蝉Empoasca vitis G?the对健康茶梢和蝉害茶梢挥发物的行为反应，筛选出可引诱假眼小绿叶蝉的茶梢挥发物。【方法】 以动态吸附法茶梢和蝉害茶梢挥发物，用气相色谱–质谱联用（GC-MS）进行定性定量分析Y形嗅觉仪挥发物假眼小绿叶蝉成虫的共鉴定出3种挥发物组分其中Z-(罗勒烯乙酸叶醇酯的含量分别是茶梢中的142.倍、12.倍。蝉害茶梢中新的有，其中紫苏烯含量较高。在Z-(-罗勒烯和DD-柠檬烯呈现出显著引诱水平（P0.05）；混合物组分Blend1和Blend2分别表现出极显著和显著引诱水平；而10-6 g/mL (-法尼烯显著排斥假眼小绿叶蝉（P0.05）。【结论】 假眼小绿叶蝉成虫对健康茶梢和蝉害茶梢挥发物多种组分具有不同的行为反应 关键词假眼小绿叶蝉茶梢Empoasca vitis G?the to volatiles from tea shoots WANG Meng-Xin(( LI Hui-Xian WU Wen-Zhu SUN Hai-Chao SHI Song-Ping DING Yuan CAO Chun-Hui HAN Bao-Yu((( (Zhejiang Provincial Key Laboratory of Biometrology and Inspection Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China) Abstract [Objectives] To determine the behavioral responses of the tea green leafhopper (Empoasca vitis G?the) to volatile compounds emitted by both intact, and leafhopper-damaged, tea shoots in order to identify volatiles that attract this pest. [Methods] Volatiles from intact and leafhopper-damaged tea shoots were adsorbed by the routine overhead entrained air method, eluted in organic solvent, concentrated under a small flow of pure nitrogen, and qualitatively and quantitatively analyzed. The responses of leafhoppers to different volatiles were assessed in a Y-tube olfactometer. [Results] Thirty components in the volatiles of both intact, and leafhopper-damaged, tea shoots were identified in which the alkene content was relatively high. A total of 13 compounds were identified in the volatiles of intact and leafhopper-damaged tea shoots. The amounts of these compounds were considerably greater in volatiles emitted by leafhopper-damaged tea shoots than those emitted by intact tea shoots, especially (Z)-(-ocimene and (Z)-3-hexenyl acetate, which were respectively 142.27 and 12.90 times higher in volatiles from leafhopper-damaged tea shoots than in those from intact tea shoots. Twelve different c