彩色马蹄莲2n配子育种技术初探-中国农学通报.PDF

中国农学通报 2011,27(8): 108-113 Chinese Agricultural Science Bulletin 彩色马蹄莲2n配子育种技术初探 1 1 1 2 李洁筠 ，吴红芝 ，陈 溪 ，周 涤 1 2 （云南农业大学园林园艺学院，昆明650201；北京市农林科学院蔬菜研究中心，北京100097） 摘 要：以彩色马蹄莲的栽培品种‘黑魔术’Black magic 、‘火烈鸟’Flamingo 为试验材料，研究彩色马蹄莲 2n 配子育种技术。选择刚从茎秆中抽出的幼小健壮小花，用0.15%的秋水仙素处理以诱导2n 配子，将诱 导的2n 配子进行不同组合的杂交实验。结果表明：秋水仙素处理对彩色马蹄莲雄雌蕊发育有一定毒副 作用，其中对雌蕊的损伤大于雄蕊；2n 配子能在一定程度上克服杂交后代不育性；在相同条件下，经2n 配子诱导处理的杂交种子与未处理的对照种子相比，种子萌发时间大幅度缩短（50 天左右），叶片变薄、 变大、叶色变浅，有些杂交植株表现出不同程度的畸变。通过对杂交后代的细胞学鉴定表明：处理配子 杂交后代中获得染色体数目为48 （2n=3x=48 ）的三倍体植株。 关键词：彩色马蹄莲；2n 配子；杂交育种；秋水仙素 中图分类号：S682.2 文献标志码：A 论文编号：2011-0287 PrimaryStudiesonBreedingTechniquesby2nGametesincoloredZantedeschiahybrid 1 1 1 2 Li Jieyun , Wu Hongzhi , Chen Xi , Zhou Di 1 ( CollegeofHorticultureandLandscape,YunnanAgriculturalUniversity, Kunming 650201; 2 VegetableResearchCenter,BeijingAcademyofAgricultureandForestrySciences, Beijing 100097) Abstract: The cultivars of colored Zantedeschia hybrid: Black magic and Flamingo are used as experimental material. The young robust buds were selected to be treated with 0.15% colchicine to induce 2n gametes. The treated young flower buds between the two cultivars were crossed when pistil and stamena matured. The results showed that: colchicine had some poison impact on the development of the pistil and androecium, especially on that of pistil; 2n gametes can overcome sterility of hybrids to a certain extent. Under the same conditions, the seed germination of hybrids derived from treated gametes was sped