ELSD应用介绍.pptVIP

ELSD应用介绍 药物、组合化学样品分析、天然药物、碳水化合物、油脂、聚合物、脂肪酸、氨基酸、表面活性剂 ELSD 2000独有双模式操作，用途更广泛 补充示差及低波长紫外的不足 质谱(MS)的理想方法开发工具 咖啡因与阿司匹林 撞击器“开”情况下使用高流速含水溶剂基线保持稳定 半挥发性样品如阿司匹林没有蒸发，灵敏度提高 短链脂肪酸 撞击器“开”情况下半挥发性脂肪酸没有蒸发，灵敏度提高 聚乙二醇 (PEG) 200 低分子量PEG 200为半挥发性 撞击器“开”情况下配合梯度洗脱，可完全分离PEG 200并得到最佳灵敏度 脂溶性维生素 撞击器“关”情况下，不挥发的脂溶性维生素有较佳检测 二甲基硅酮 撞击器“关”情况下已可分离及检测不挥发的二甲基硅酮 此方法用的氯仿因为末端吸收问题不能用UV，而梯度洗脱也排除了RI的使用 含氮肥料 样品中原预计尿素是DMTU浓度七倍 ELSD比UV给出较接近实际的样品组成 溴化阻燃剂 四溴双酚A比六溴环十二烷含更多紫外吸收官能团 溴化阻燃剂分析 六溴环十二烷含量为四溴双酚A三倍 ELSD正确显示两者比例为71：29，而UV峰面积比则为15：85 * * # 8475 # 8476 1. 月桂酸 - 0.3mg/ml 2.肉豆蔻酸 - 0.3mg/ml 3.十五烷酸 - 0.3mg/ml 4.棕榈酸 - 0.3mg/ml # 8491 # 8492 # 8618 # 8620 # 8619 # 8636 # 8623 # 8624 1. 尿素 2. 二亚甲基二尿素 3. 二亚甲基三尿素(DMTU) # 7869 # 7872 This method uses higher flowrate with 50% water, and impactor on mode achieves a more stable baseline under these conditions. Impactor On is more sensitive especially for the low MW fatty acids of lauric and myristic acid. Next is another example of an ideal Impactor On method Here is an example where Impactor Off is ideal. The combination of mostly organic mobile phase and non-volatile sample composition makes Impactor Off best for this method. ? Dimethicone or polysiloxane is a silicone derived oil commonly used in the cosmetics industry as a spreading agent and moisturizer ? It is a difficult sample to analyze because it contains no UV chromophore and depending on viscosity can have limited solubility Urea is a semi-volatile compound so Impactor on operation Add in more information about sample * * * This method uses higher flowrate with 50% water, and impactor on mode achieves a more stable baseline under these conditions. Impactor On is more sensitive especially for the low MW fatty acids of lauric and myristic acid. Next is another example of an ideal Impactor On method Here is an example where Impactor Off is ideal. The combination of mostly organic mobile phase and non-volatile sample composition makes Impactor Off best for this method. ? Dimethicone or