2种方法制备银杏内酯长循环固体脂质纳米粒的比较.pdf

2种方法制备银杏内酯长循环固体脂质纳米粒的比较 刘 旻，陈建海，董芙蓉，刘 园(南方医科大学南方医院药学部，广州市 510515) 中图分类号 R283；R94 文献标识码 A 文章编号 1001-0408(2008)12-0905-03 摘 要 目的:研究银杏内酯 AB长循环固体脂质纳米粒(GAB-LSLN)的制备方法，并探讨 GAB-LSLN的主要理化性质。方法:分别采用超声法和高压乳匀法制备GAB-LSLN。在电镜下观 察其形态，测定其粒径、Zeta电位和包封率，并在室温下放置4周，观察GAB-LSLN的稳定 性。结果:超声法制备的GAB-LSLN在透射电镜下呈片状存在，形态不规则；高压乳匀法制备 的GAB-LSLN呈球状，形态规则。 超声法和高压乳匀法制备的GAB-LSLN粒径分别为(219.6 14.3)nm和(173.910.4)nm(P0.001)；Zeta电位分别为(21.121.03)mv和( 27.432.14)mV(P0.001)，包封率分别为(85.050.67)%和(92.490.88)%(P0.001)。高 压乳匀法制备的GAB-LSLN室温放置4周后，粒径无显著增加(P0.05)。结论:高压乳匀法制 备GAB-LSLN具有粒径小、稳定性和包封率高的特点，优于超声法。 关键词 银杏内酯A、B；超声法；高压乳匀法；固体脂质纳米粒；长循环 Ultrasonication VersusHigh PressureHomogenization in thePreparation of GinkgolidesAandBLong-circulatingSolidLipidNanoparticles LIUMin，CHENJian_hai，DONGFu_rong，LIUYuan(Dept.ofPharmacy，NanfangHospital， SouthernMedicalUniversity， Guangzhou510515， China) ABSTRACT OBJECTIVE:Tostudythepreparativemethodsofthelong-circulatingsolid lipidnanoparticles(LSLN)car-ryingginkgolidesAandB(GAB)andtostudythe physicochemicalcharacteristicsoftheGAB-LSLN.METHODS:GAB-LSLNwaspreparedby ultrasonicationorhighpressurehomogenization.Transmissionelectronmicroscopy wasemployedtostudyitsshape.Particlesize， zetapotential， andentrapment efficiencyofGAB-LSLNweredetermined， anditsstabilityafterstorageunderroom temperaturefor4weekswasdeterminedaswell.RESULTS:TheGAB-LSLNpreparedby ultrasonica-tionwasplatelet-shapedandirregular， andthatpreparedbyhigh pressurehomogenizationwassphericalandregularinshape.Theparticlediameters ofGAB-LSLNpreparedbyultrasonicationandhighpressurehomogenizationwere (219.614.3)nmand(173.910.4)nmrespectively(P0.001)； theZetapotentials were(21.121.03)mvand(27.432.14)mvre-spectively(P0.001)； the entrapment efficiencies were(85.05 0.67)% and(92.49 0.88)%(P0.001) respectively.GAB-LSLNpreparedbyhighpressurehomogenizationshowednoobvious increase inparticlesizeafter storageatroom tem-peraturefor4weeks (P0.05).CONCLUSION:Highpressurehomogenizationissuperiortoultraso