男性不育患者解脲支原体、沙眼衣原体感染及其对精液质量的影响.docVIP

精品论文 参考文献 男性不育患者解脲支原体、沙眼衣原体感染及其对精液质量的影响 杨爱景 　　【摘 要】 目的 探讨男性不育患者解脲支原体（UU）、沙眼衣原体（CT）感染情况及其对精液质量的影响。方法 采用荧光定量聚合酶链反应（FQ-PCR）对330例男性不育患者（不育组）、148例正常生育男性(生育组)的精液进行UU、CT检测及精液常规分析。结果 不育组UU、CT总检出率为 49.39 %，显著高于对照组 14.86 %（x2= 49.26, plt;0.01）；其中单纯UU检出率为39.09% 、单纯CT检出率为16.36 %，明显高于生育组12.16 %、3.38%（x2= 34.79，plt;0.01； x2=13.92，plt;0.01）；不育组UU、CT两者同时感染20例，检出率为6.06%，不育组UU检出率显著高于CT（x2= 42.53，plt;0.01 ）。而感染UU和/CT阳性的患者精子的密度、、活动率、活力明显低于阴性组，精子的畸形率、液化时间、白细胞数明显高于阴性组，差异有统计学意义（Ｐ＜0.05）。结论 UU和CT感染对精子多项指标有影响，精液质量下降，是引起男性不育的重要因素之一，对男性不育患者应常规检测UU和CT。 　　【关键词】 解脲支原体； 沙眼衣原体； 精子质量； 男性不育； 荧光定量聚合酶链反应； 　　Male infertility patients with Mycoplasma urealyticum, Chlamydia trachomatis infection and its effect on semen quality. Yang Aijing.The Clinical Laboratory of Shandong Heze Municipal,Hospital,Heze 274000 China. 　　【Abstract】 Objective Study of male infertility Ureaplasma urealyticum (UU), Chlamydia trachomatis (CT) infection and its effects on semen quality. Methods 330 cases of male infertility (infertility group) were detected by fluorescence quantitative polymerase chain reaction (FQ-PCR), UU, CT and semen routine analysis were performed in 148 normal fertile men (Group). Results The total detection rate of UU and CT was 49.39% in sterility group,it was significantly higher than 14.86% in control group(x2=49.26,plt;0.01);The detection rate of UU、CT was 39.09%、16.36% respectively in sterility group and they were significantly higher than 12.16%、3.3800% in control group(x2=34.79,plt;0.01; x2=13.92,plt;0.01);17 sterile men were infected with UU and CT at the same time,the positive rate was 6.06%. The detection rate of UU was sighificantly higher than that of CT(x2=42.53,plt;0.01) in sterility group. And infected UU and / CT positive patients with sperm density, activity rate, vitality was significantly lower than the negative group, the abnormal rate of sperm, liquefaction time, the number of white blood cells was significantly higher than the negative group, the difference was s