HPLC离子检测法测定心脏停跳液中主要成份的含量研究.pdfVIP

HPLC--离子检测法测定心脏停跳液中主要成份的含量 张正 郑英丽 陈宝玲 (中国医学科学院 阜外心血管病医院药剂科 北京 100037) 摘要 目的 建立同时测定心脏停跳液中钾 钙 镁含量的离子检测法 方法 采用Alltech Universal Cation 色谱柱 (100x4.6mm), 柱温为35ºC, 5mMHCL 为流动相 流速为 1.0mL/min 结果 钾离子在 12.00~28.00µg/mL 范围内 峰 面积与其浓度线性关系良好 r=0.999 8 平均回收率为 101.1 重复性试验 RSD 为 1.59 n=6 镁离子在 12.00~28.00µg/mL 范围内 峰面积与其浓度线性关系良好 r=0.999 7 平均回收率为 101.1 重复性试验RSD 为 1.94 n=6 钙离子在 2.40~5.60µg/mL 范围内 峰面积与其浓度线性关系良好 r=0.992 5 平均回收率为 100.8 重复性试验RSD 为2.00 n=6 结论 本方法准确 简便 快速 有希望用于停跳液的质量控制 关键词 离子检测法 停跳液 质量控制 中图分类号 文献标识码 文章编号 HPLC-ION measuring of the concentrations of the main components in cardioplegic solution ZHANG Zheng, ZHENG Ying-li, CHEN Bao-ling (Department of Abstract Objective : To establish a HPLC for measuring the concentrations of the main components in cardioplegic + 2+ 2+ solution . Method: To determine the contents of K , Ca , Mg in the cardioplegic solution by HPLC with an Alltech Universal Cation column (100x4.6mm) and ion detecting instrument . The mobile phase was 5mM hydrochloric acid, the flow rate was 1.0 mL/min. Results: Calcium, magnesium and procaine in cardioplegic solution can be separated . The linear correlation of the potassium calibration curve was excellent (r=0.999 8) while the concentration of potassium was in the range of 12.00~28.00µg/mL , and the average recovery was 101.1% with repeatability precision RSD of 1.59%(n=6) . The linear correlation of the magnesium calibration curve