Microparticle 3D transportation on the membrane - 投影片 1.pptVIP

Microparticle 3D Transportation on the Membrane A gold Nanoparticle Coated by Bovine Serum Albumin on the Membrane Observation of Cancer Cell Filopodia without Staining * * The 1-mm bead is coated with fibronectin, which links to actin filaments through integrins. From the motion of the bead we calculated that the treadmill speed of the actin on the filament is ~ 17 nm/min, while the elongation rate of the actin branch is ~ 8 nm/min. C.-C. Wang, J.-Y. Lin, H.-C. Chen, and C.-H. Lee, Optics Letters 2006, 31, 2873 Actin branch elongation Treadmill movement of actin on the filament Lateral movement of actin filament Lateral movement of the bead 3D movement of the bead m 600 400 200 0 nm 10 mm NIWOP topography GFP-actin image Actin aggregates around the binding site of the gold particle (dia. = 80 nm). The nanoparticle moved into the membrane by ~ 400 nm, and then was expelled out, which indicates that the endocytosis of this particle did not complete. C.-C. Wang, J.-Y. Lin, C.-W. Lee, C.-Y. Huang, P.-K. Wei, and C.-H. Lee, Applied Optics 2008, 47(13), 2458-2464 Bright field + Restoration Bright field NIWOP NIWOP + Restoration 2 mm 10 mm lung cancer cell T.-H. Hsu, W.-Y. Liao, P.-C. Yang, C.-C. Wang, J.-L. Xiao, and C.-H. Lee*, Optics Express 2007, 15, 76 Filopodia are on the bottom of the cell. bright field confocal fluorescence Lateral resolution is improved by using a maximum likelihood estimation algorithm to 130 nm. The filopodia can be observed clearly. filopodium * *