semiquantitative immunohistochemistry for mucin(muc1,muc2,muc3,muc4,muc5ac,and muc6)profiling of pancreatic ducta.半定量的免疫组织化学粘蛋白(muc1、muc2 muc3,m.pdf

Histopathology 2016, 69, 582–591. DOI: 10.1111/his.12994 Semiquantitative immunohistochemistry for mucin (MUC1, MUC2, MUC3, MUC4, MUC5AC, and MUC6) profiling of pancreatic ductal cell adenocarcinoma improves diagnostic and prognostic performance Marek Sierzega, Damian Młynarski,1 Romana Tomaszewska1 Jan Kulig First Department of Surgery, and 1Department of Pathology, Jagiellonian University Medical College, Krakow, Poland Date of submission 2 December 2015 Accepted for publication 6 May 2016 Published online Article Accepted 11 May 2016 Sierzega M, Młynarski D, Tomaszewska R Kulig J (2016) Histopathology 69, 582– 591. DOI: 10.1111/his.12994 Semiquantitative immunohistochemistry for mucin (MUC1, MUC2, MUC3, MUC4, MUC5AC, and MUC6) profiling of pancreatic ductal cell adenocarcinoma improves diag- nostic and prognostic performance Aims: Mucin (MUC) glycoproteins are involved in were significantly higher for PDAC, whereas patients various steps of the carcinogenesis and progression of with non-malignant tissues had higher values for human malignancies. The aim of this study was to MUC3 and MUC6. The area under the curve for the verify whether semiquantitative evaluation of MUC ROC curve derived from the diagnostic model includ- staining by immunohistochemistry may help to differ- ing MUC3, MUC5AC and MUC6 was 0.96 [95% con- entiate pancreatic ductal cell adenocarcinoma (PDAC) fidence interval (CI) 0.91–0.98], with 85% sensitivity from chronic pancreatitis and normal pancreas. and 94% specificity. Median QS values for MUC2 Methods and results: Mucin expression was examined were significantly higher in patients with less by immunohistochemistry in surgical specimens advanced