cOmpleteHisTagPurificationResin中文指导教程.pdf

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2018-04-14 发布于江苏
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cOmpleteHisTagPurificationResin中文指导教程.pdf

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cOmplete His-Tag Purification Resin 说明书版本01 2012 年5 月即用型的镍离子螯合树脂，用于小规模至大规模的His 标签融合蛋白纯化 25ml 包装目录号：05 893 682 001 200ml 包装目录号：05 893 801 001 保存于：+2～+8℃ 1 1．产品用途 ························································3 1.1 介绍 ···································································3 重组蛋白表达 ··························································3 通过固定化的Ni2+离子纯化蛋白 ············································3 His-Tag ································································3 1.2 试剂特性 ·····························································3 技术参数 ·······························································4 1.3 试剂组成 ······························································5 1.4 保存和稳定性 ··························································5 1.5 应用 ··································································5 2 ．产品使用方法 ···················································6 2.1 纯化注意事项··························································6 选择合适的宿主微生物 ···················································6 选择天然或变性条件下的纯化 ·············································6 选择合适的His 标签长度 ··················································6 选择合适的缓冲系统 ·····················································6 裂解液制备 ·····························································6 2.2 实验前注意事项·······················································7 2.3 柱层析纯化流程 ·······················································7 FPLC 纯化的装柱流程 ·····················································7 天然条件下的蛋白纯化流程 ···············································7 变性条件下的蛋白纯化流程 ···············································8 2.4 批量纯化流程 ··························································9 2.5 清洗流程 ·····························································10 天然条件的柱清洗 ··························