材料与方法1.doc

改良LPD液、硝普钠对犬肺缺血再灌注损伤ET-1的影响△ 王磊☆,刘公哲,齐海★,李元明,高双庆,马爱国 （新疆医科大学第二附属医院胸外科 新疆 乌鲁木齐 830000) 【摘要】目的：采用犬单肺原位常温缺血再灌注模型，探讨及研究改良LPD液（Low potassium dextra glucose,LPD），改良LPD液+硝普钠两种保护液对犬肺缺血再灌注损伤时血浆及肺组织匀浆中内皮素-1（Endothelin-1,ET-1）的影响有无差异。方法：选用健康成年杂交犬12只， 随机分成两组，每组6只，A组以常温LPD液行左肺灌洗，灌注量50ml/kg～60ml/kg，灌注压力40mmH2O；B组以常温LPD液+硝普钠行左肺灌洗，其余同A组。两组采用酶联免疫吸附法（Enzyme-linked immuno sorbent assay，ELISA）分别于再灌注后0h、2h、4h测定血浆、肺组织匀浆中ET-1的含量以及实验结束时称取肺湿、干重，计算肺湿/干比重。结果：两组血浆、肺组织匀浆中的ET-1含量无差异（p0.05），肺湿/干比重比较有变化（p0.05）。结论：改良的LPD液可能通过减少ET-1的表达水平，降低肺湿/干比重，减轻肺缺血再灌注损伤，但在改良的LPD液基础上加上硝普钠，对ET-1的表达水平及肺湿/干比重无明显影响。 【关键词】改良LPD液；硝普钠；肺缺血再灌注损伤；ET-1；肺 【中图分类号】 R655.3 【文献标识码】 A 【收稿日期】2012-06-10 Effects of modified low potassium dextran solution ,sodium nitroprusside on endothelin-1 during ischemia-reperfusion injury in dogs△ WANG Lei☆,LIU Gongzhe ,QI Hai★,LI Yuanming,GAO Shuangqing,MA Aiguo （Second Affiliated Hospital,Xinjiang Medical University,Urumqi830028,China） 【Abstract】ObjectiveTo study effects of modified low potassium dextran solution , sodium nitroprusside on endothelin-1 , situ single-lung ischemia-reperfusion injury model of dogs were explored. Methods：A total of 12 healthy adult hybrid dogs were randomly divided into two groups with six animals in each. Group A , left lung were flushed with 50ml/kg ~ 60ml/kg of hepatic modified low potassium dextran solution at a pressure of less than 40mmH2O. Group B ,were flushed with hepatic modified low potassium dextran solution and sodium nitroprusside,other same as group A. The change of ET-1 content in hepatic tissue and serum after reperfusion of left lung for 0, 2 and 4 hours were observed by enzyme-linked immunosorbent assay (ELISA) , in the end of the experiment calculated the variation of lung wet/dry ratio . Results：The ET-1 contents of hepatic tissue and serumin no significant changes (p0.05),and wet/dry ratio in two groups has significant changes（p0.05）Conclusion：When ischemia-reperfusion injury were flushed with modified low potassium dex