HPLC同时测定冠心康颗粒中芍药苷、丹酚酸B和羟基红花黄.PDF

HPLC 同时测定冠心康颗粒中芍药苷、丹酚酸B 和羟基红花黄色素 A 的含量  喻录容，何先元* ，黄英如(重庆医科大学中医药学院，重庆 401331) 摘要：目的 建立同时测定冠心康颗粒中芍药苷、丹酚酸 B 和羟基红花黄色素 A 含量的方法。方法 采用 Welchrom C18 1 色谱柱(250 mm ×4.6 mm ，5 μm) ，以乙腈(A)-0.1%磷酸(B)为流动相，梯度洗脱；流速：1.0 mL·min ；检测波长：230 nm 。 结果 芍药苷在 7.02~52.64 μg·mL1 内呈良好的线性关系(r=0.999 7) ，平均回收率为 99.3% ，RSD 为 1.2%；丹酚酸 B 在 23.19~173.90 μg·mL1 内呈良好的线性关系(r=0.999 7) ，平均回收率为 98.2% ，RSD 为 1.1%；羟基红花黄色素 A 在 4.47~ 33.50 μg·mL1 内呈良好的线性关系(r=0.999 3) ，平均回收率为 98.0% ，RSD 为 1.0%。结论 本法简便、灵敏、准确、重 复性好，可用于冠心康颗粒的质量控制。 关键词：冠心康颗粒；芍药苷；丹酚酸 B ；羟基红花黄色素 A ；高效液相色谱法 中图分类号：R284.1 ；R917.101 文献标识码：A 文章编号：1007-7693(2015)05-0561-04 DOI: 10.13748/ki.issn1007-7693.2015.05.010 Simultaneous Determination of Paeoniflorin, Salvianolic Acid B and Hydroxysafflor Yellow A in Guanxinkang Granules by HPLC * YU Lurong, HE Xianyuan , HUANG Yingru(Institute of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 401331, China) ABSTRACT: OBJECTIVE To establish a simultaneous determination method for paeoniflorin, salvianolic acid B and hydroxysafflor yellow A in Guanxinkang granules. METHODS The determination was performed on a Welchrom C18 column(250 mm×4.6 mm, 5 μm) with acetonitrile(A)-0.1% phosphoric acid(B) as the mobile phase in gradient elution mode at the flow rate of 1.0 mL·min1. The detection wavelength was 230 nm. RESULTS Paeoniflor presented a good linearity in the concentration range of 7.0252.64 μg·mL1. The average rate of recovery was 99.3 % with RSD of 1.2%. Salvianolic acid B presented a good linearity in the concentration range of 23.19173.90 μg·mL1. The average rate of recovery was 98.2% with RSD of 1.1%. Hydroxysafflor yellow A presented a good linearity in the concentration range of 4.4733.