官方说明书大鼠抗酒石酸酸性磷酸酶5b定量测定试剂盒(酶联免疫法) 说明书.pdf

5b SB-TR201A 5b Rat TRACP 5b Assay Kit ELISA 5b TRAP5b TM RatTRAP test TRAPTRAP TRAPTRACP ——5a5bTRAP RatTRAP™TRAP 5bRatTRAP™ TRAPTRAPRatTRAP™ RatTRAP™, (0)1,2,4,7 14 TRAP 5b , 2 TRAP 5b RatTRAP™RatTRAP IgGTRAP 5b TRAP 5aTRAP 5bPH TRACP 5b 1CAL 1-4 – REF SB-TR102 01A - SB-TR102 01D: Tris-RatTRAP0.09% 0.5 mL4 2Ab – -TRAP ( REF SB-TR102 02): Tris--TRAP0.05%10 mL 3MICROPLAT – ( REF SB-TR102 02W) : IgG8X12 4CTRL – ( REF SB-TR102 05) : Tris-RatTRAP0.09% 0.5 mL 5NaOH – ( REF SB-TR000 06): 0.32M6 mL 6. RELEASREAG – ( REF SB-TR102 07): TRAP6 mL 7. SUBS pNPP – ( REF SB-TR000 08): pNPP 2 1 100 µL-RatTRAP Ab MICROPLAT . ↓ 60@ 20-24°C ↓ ↓ 100 µL CAL CTRL MICROPLAT . 75 µL 0.9% NaCl25 µL MICROPLAT . 25 µL RELEASREAG ↓ 60 minutes @ 20-24°C ↓ ↓ 100 µL ↓ 2 hours @ 37°C. ↓ 25 µLNaOH ↓ @ 405nm. U/L RatTRAP(1:4)20 µL80 µL0.9% NaCl5 - U/L A1, A2 Blank 0.190 0.190 -