细胞转分化技术研究进展-中国细胞生物学学报.PDF

网络出版时间：2013-05-09 15:21 网络出版地址：/kcms/detail/31.2035.Q1521.003.html 中国细胞生物学学报 Chinese Journal of Cell Biology 2013, 35(6): http ://www.cj 细胞转分化技术研究进展 * * 马奎莹 范安然 谭文涛 张学明 李子义 (吉林大学动物医学学院, 动物胚胎工程吉林省重点实验室, 长春130062) 摘要 随着体细胞核移植、细胞融合及细胞转分化等技术的发展与完善, 现已证明多数动物 细胞的细胞核具有全能性, 高度分化细胞的命运也并非是不可逆的。特定的细胞类型在合适的重 编程转录因子诱导下可转分化为另一细胞类型。转分化技术为人类细胞疾病模型和分子替代疗法 提供了一个崭新的思路。该文评述了近年来将成纤维细胞直接或间接转分化为其他细胞类型的相 关进展, 就该领域目前存在的问题和挑战及其临床潜在应用价值进行了讨论, 以期为相关研究提供 借鉴。 关键词 转分化; 直接转分化; 间接谱系转化; iPSCs The Recent Progress of Cellular Transdifferentiation Ma Kuiying, Fan Anran, Tan Wentao, Zhang Xueming, Li Ziyi* (Jilin Province Key Laboratory of Animal Embryo Engineering, College of Animal Veterinary Medicine, Jilin University, Changchun 130062, China) Abstract With the development of techniques including somatic cell nuclear transfer, cell fusion and cellular reprogramming, mass of data indicate that most of animal cell nuclei have totipotency or pluripotentency. Thus, the fate of terminal differentiated cells is not absolutely irreversibly. Recently, it is reported that differentiated cell can be transdif- ferentiated into another cell type using appropriate transcription factors. This innovative transdifferentiation strategy pro- vides a new approach for human disease models and molecular therapy. Aiming to provide useful references for related studies in this field, we summarize the latest progress of direct/indirect transdifferntiation of fibroblasts, and