ascl2mir200zeb轴调控结肠癌细胞emtmet的可塑性分析word格式论文.docx

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ascl2mir200zeb轴调控结肠癌细胞emtmet的可塑性分析word格式论文.docx

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ascl2mir200zeb轴调控结肠癌细胞emtmet的可塑性分析word格式论文

mlmilliliter毫升mRNAmessengerRNA信使RNAMTTMethylthiazolyltetrazolium甲基噻唑基四唑ODOpticaldensity光密度PBSPhosphatesalinebuffer磷酸盐缓冲液PCRPolymerasechainreaction聚合酶链反应PVDFPolyvinylidenedifluoride聚偏二氟乙烯rpmRevolutionsperminute每分钟转数RNARibonucleicacid核糖核酸RT-PCRReversetranscriptasePCR反转录PCRSDSSodiumdodecylsulfate十二烷基硫酸钠shRNAShorthair RNA短发卡RNATBSTris-bufferedsalineTris-HCl缓冲盐溶液TBSTTrisbufferedsalinewithTween20含Tween20 的TBS 缓冲液TEMEDTetramethylethylenediamine四甲基乙二胺Modulationoftheplasticitybetweenepithelialand mesenchymalfeaturesofcoloncancercellsby Ascl2/miR-200/ZEBaxisAbstractBackground:Colon cancerisoneofthecommongastrointestinalcancers,withpoorprognosisandahighermortalityrate.Itistheworld’smortalitycameinthirdplace,thefirstofmorbidityandmortalityindevelopedcountries.Thetreatmentofcoloncancerhasbeenamajorproblem,whichistheexistingmethodsoftreatment,includingsurgery,chemotherapy,butdidnotchangethenaturaloccurrenceofcoloncancer.Westillfacetheenormous challengesbecauseofitslowrateofearlydiagnosisandthetreatmentofcolorectalcancerinlatestage.EMT(epithelial-mesenchymaltransition,EMT)hasbeenfoundtoexistinmechanismof themajoritiesofmalignant tumor,such asbreast, colon andprostatecancers.TheabnormalactivationofEMTinhumantumorcelllinesandanimalmodelsconfirmed thatthemechanism isassociatedwiththetumorcellsdisseminationandcirculationintothesurroundingtissue,thustheEMTisalsoconsideredtobeaveryimportantmechanismof tumorinvasionandmetastasis.AlargeamountofliteraturehasconfirmedthatmiRNA-200 familyisinvolvedintheregulationoftheEMTthroughinteractingwithZeb1/2byanegativefeedback.ButtheregulationofmiRNAsexpressionbythetranscriptionalmechanismorother mechanismshas notbeenwidelyrecognized.Ascl2(Achaetescute-like2)isabasicbHLH(helix-loop-helix)transcriptionfactor,whocontrolsthefateofintestinalstemcellsand coloncancer stemcells bya miR-302b-relatedmechanism.Stillthe effortsinstudyingEMTandstemcellproperties,regulatingoftheplasticityEMT-stemnessisnotclearandthemechanismsthatregulatemiR-200familyisalsolargelyu